ShopGentleness in Cell Sorting with the WOLF G2
Healthy Cells. Broad Capabilities. Better Science.
High cell integrity without compromise
When used with the N1 Single-Cell Dispenser, the WOLF G2 allows for single-cell sorting into 96- or 384-well plates. Its versatility and enhanced functionality make it an ideal tool for various applications, including stem cell, single-cell genomics, cell line engineering, gene editing, antibody discovery, immunology, infectious disease, plant biology, basic research, and more.
Keep Your Cells Happy
Application Overview
The NanoCellect WOLF G2 cell sorter is a powerful tool used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The WOLF G2 is designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The WOLF G2 cell sorter is ideal for tasks like cell line development and sorting plant protoplasts, as well as for monoclonal outgrowth of iPSCs and microalgae.
Microfluidic Cartridge Technology
- Unique to NanoCellect are our disposable cartridges that allow for bulk sorting or single-cell sorting.
- The sorting cartridges use a piezoacoustic actuator that gently directs cells into collection channels; an embedded cell sorting verification system gives instant feedback of sorting accuracy.
- Sort two selected cell populations with bulk sorting while the remainder of cells collects in a third channel.
- Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser.
WOLF G2 Specifications
| Sample input | 1.5, 2.0, and 5.0 mL tubes |
| Sheath input | 50 mL conical tubes |
| Sheath fluid | PBS or buffer of choice |
| Sheath fluid usage | 9.6 mL/hour |
| Sample flow rate | 24 μL/minute |
| Sheath flow rate | 160 μL/minute |
| Sample line volume | 50 μL |
| Minimum sample volume | 100 μL |
| Tubing diameter (inner) | 200 to 500 μm |
| Flow cell | 200 x 700 μm |
| Smaller channel diameter | 70 μm |
| Sample pressure | < 2 psi |
| Sample output (bulk sorting) | 1.5 mL or 5 mL tubes |
| Sample output (single cell) | 96- or 384-well plates (flat/U/V bottom or PCR) |
| Time to plate (96 wells) | 3 - 8 minutes |
| Time to plate (384 wells) | 32 minutes |
| Droplet volume | 3 - 10 μL |
| Sample plate options | 96 or 34 well (flat bottom, V-bottom, U-bottom, PCR) |
| Scatter sensitivity | < 1.5 μm by FSC or BSC |
| Scatter resolution | Resolves lymphocytes, monocytes and granulocytes |
| Fluorescence sensitivity | < 200 MESF FITC (488 nm laser)< 250 MESF PE (561 nm laser) |
| Fluorescence resolution | 9-peak separation with SPHERO Rainbow Calibration Particles |
| Analysis speed | 2,000 events/second |
| Sorting | 1- and 2-ways |
| Back-to-back sorting speed | 200 events/second |
| Absolute counts | Yes |
| Volumetric counts | Yes |
| Warm-up time | Less than 1 minute |
| Sorting purity | Up to 99% purity |
| Laser Profile | 20 x 90 μm |
| Scatter detection | Forward (0 degrees, +/- 15) Back (180 degrees, +/- 15) |
| Excitation & emission detection | See WOLF G2 Configuration Guide |
| Optical alignment | Fixed alignment No maintenance required |
| WOLF G2 Dimensions | 14.8H x 18.0W x 13.6D inch (37.6H x 45.8W x 34.5D cm) |
| WOLF G2 weight | 54 lbs / 24.5 kg |
| WOLF G2 Electrical | AC Input: 100-240V, 50-60Hz, 2A |
| N1 Dimensions | 8.42W x 6.5H x 8.34D in (21.4 x 16.5 x 20.9) |
| N1 Weight | 5.5 lbs / 2.5 kg |
| N1 Electrical | DC Input: 24V, 1A |
Accessories and Software
N1 Single-Cell Dispenser
Designed to sort and dispense into 96- and 384- well plates, the N1 provides higher rates of singlet detection compared to cell printers or limiting dilution. Users can perform simple, label-free dispensing or advanced multicolor panel single-cell dispensing.
Plate sorting specifications
Time to plate (96 wells): 3 - 8 minutes
Time to plate (384 wells): 32 minutes
Droplet volume: 3 - 10 µL
Sample plate options: 96 or 384 wells (flat bottom, V-bottom, U-bottom, PCR)
CS1 Chiller-Stirrer Accessory
Applications with temperature sensitive samples, such as Stem cells or Neuronal cells can be challenging to sort. Such applications would benefit from modules such as the CS1 Chiller-Stirrer by keeping both sheath fluid and sample at the desired temperature during the sorting process.
WOLFViewer Software
The WOLFViewer software has an intuitive workflow
menu that walks users through their experimental
process, and is designed for both novice and expert
users. New users can be performing their first sorts in
about 20 minutes and can be on their own on day one.
Plate sorting specifications
System power up: 3 minutes
System setup and calibration: 20 minutes
System shutdown: 3 minutes
Additional features: Auto-alignment, intuitive compensation, advanced coloring and gating options, FSC files compatible with FlowJo
WOLF G2 Configuration
The WOLF G2’s dual laser configuration allows 9 fluorescent colors, it empowers researchers with expanded capabilities while maintaining user-friendly workflows for bulk sorting or precise single-cell dispensing.
Flow Cytometry with the WOLF G2:
Gentleness, and Efficiency
The WOLF G2 is a game-changer for flow cytometry, offering unparalleled gentleness in cell sorting. Its advanced microfluidic technology ensures minimal cell stress and high viability, making it ideal for delicate applications such as stem cell research and immunology. With dual-laser capabilities and up to nine fluorescence channels, the WOLF G2 provides robust, high-resolution data for complex analyses. The easy-to-use interface and compact design allow researchers to conduct sophisticated flow cytometry experiments directly on their benchtop, streamlining workflows and accelerating discoveries.
How Does Flow Cytometry Work?
Footnotes:
† Graphics created with Biorender