Single Cell Genomics
The field of genomics and computational biology has progressed immensely over the last decade. The different types of genomic approaches and techniques have advanced our understanding of human disease and many other biological problems that face the world. Within the field of genomics, sample preparation is a significant yet underappreciated step that allows for high-quality sequencing and analysis, critical to achieving breakthroughs in medicine, agriculture, and other industries.
NanoCellect’s microfluidic cell sorter, the WOLF®, allows the cell’s integrity to be maintained for numerous sequencing and multi-omics techniques. The WOLF’s ability to flexibly fit into any genomic researcher’s lab, make it a key step to any cell sequencing analysis workflow.
Single Cell genomics has revolutionized the sequencing space by being able to decipher cell-to-cell differences from a large and diverse starting population of cells. For systems biology study of single-cell variations, such as immune cell populations, microfluidic-based cell sorting can allow users to sort a specific group of cells to complete single-cell or spatial views of genomics. The sorted population can be loaded into a partitioning library prep based platform, like 10x Genomics RNA-seq or other single-cell transcriptome technologies. By sorting upstream of single-cell genomic-based experiments, users will achieve better resolution of their cells of choice by eliminating wasted reads on dead cells or debris. Researchers don’t need to worry about cell sorting affecting the stress of cell health and thus influencing cellular RNA—the WOLF operates at a low, gentle pressure maintaining endogenous expression profiles.Learn More
Single-Cell sorting on PCR plate
Some researchers need to complete 3’ transcriptome NGS on populations of cells or on single-cells. In the case of Single-cell 3’ RNA-seq, the WOLF and N1 are especially well suited to gentle deposition into PCR plates. Utilizing the WOLF and N1’s capability of employing flow cytometry-based cell sorting, researchers can dispense single-cells of interest into 96- or 384-well PCR plates in order to complete library preparation. 3’ RNA-seq allows users the flexibility and convenience of single-cell analysis by tagging individual cells for library construction to be performed in a single tube. For example, users can complete tissue dissociation of a tumor sample, and study the heterogeneity of progenitor or tumor cells by using 3’ RNA-seq. In combination with the WOLF and N1 single-cell dispensing, researchers have a flexible, cost-efficient, and simple method to complete single-cell transcriptome analysis.Learn More
Scientists will be able to complete varying types of whole-genome sequencing methods by easily sorting for specific populations by bulk sorting on the WOLF. Researchers can sort cells with extremely large genomes, like human, plant, or animal, and learn a variety of information regarding population genetics or disease research. Users also can complete small genome sequencing by sorting for microorganisms like yeast and bacteria. By bulk sorting for a variety of cell types, genomics researchers can complete other whole genome techniques like de novo and phased sequencing, where reference genomes may not be available, or the comparison of alleles may be critical for genetic disease studies. The WOLF allows labs to be completely flexible when it comes to sequencing research and eliminate carryover with a 100% disposable fluidic cartridge.Learn More
By sorting away dead cells or debris using flow cytometry on a microfluidic cell sorting platform, users will be left with a high-quality sample that can be prepared for an ATAC-seq assay. ATAC-seq detects open chromatin regions and allows researchers to analyze chromatin accessibility at a population-wide or single-cell level. The WOLF prepares samples of high quality such that specialty genomic techniques like ATAC-seq can be easily be accomplished. The epigenomic landscape of many different populations of cells, or single-cells, can be elucidated simply by completing sample prep with the WOLF cell sorter.Learn More
A great machine that has helped us take our cell-engineering pipeline to the next level!RONAN O'CONNELL Rice University