WOLF and WOLF G2 Cell Sorter Accessories

CS1 Chiller-Stirrer Accessory

Applications with temperature sensitive samples, such as Stem cells or Neuronal cells can be challenging to sort. Such applications would benefit from modules such as the CS1 Chiller-Stirrer by keeping both sheath fluid and sample at the desired temperature during the sorting process. This allows for a more desirable environment for temperature sensitive cells and increases viability of the sorted samples. Additional to the cooling mechanism, the CS1 Chiller-Stirrer can provide stir function for samples that settle quickly or aggregate. This will maintain single-cell suspension which will improve sort recovery and purity.

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N1 Single-Cell Dispenser Accessory

A module for the WOLF Cell Sorter to sort and dispense into 96-well or 384-well plates.

Applications requiring the isolation, sorting and dispensing of single cells, such as B-cell identification for antibody discovery, or monoclonal CHO cell line development, are now easier with the N1 Single-Cell Dispenser. Additionally, single-cell genomics assays can be performed where single cells are plated directly into PCR plates.

Users can select one population of cells for single-cell deposition into 96- or 384-well plates or choose two different populations of cells for bulk cell sorting. Each well of a 96- or 384-well plate can receive anywhere from 1 to 100 cells as they are sorted from the sample.

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The N1 Single Cell Dispenser is seamlessly integrated into the intuitive software. Select single-cell deposition mode, map out your plate, and the instrument is ready to go. The WOLF cell sorter and dispenser work together to deliver plated cells in a compact and affordable format. After sorting, data from each cell is indexed well-by-well, allowing for confirmation of the plated cell.

The N1 single-cell dispenser is ideal for a multitude of applications including single cell cloning and works seamlessly with the microfluidic-based WOLF cell sorter for easy single cell isolation, single cell analysis, and cell line development. The gentle low-pressure sorting mechanism and single cell manipulation preserve cell viability through the sorting process and provide higher outgrowth of healthy clones. Reduced stress via gentle microfluidic cell sorting (low throughput) process also avoids potential adverse gene expression or karyotype changes experienced with a traditional cell sorter.

The WOLF uses a small fraction of the sheath fluid used by traditional sorters, allowing users to use growth media as sheath. Using growth media as sheath greatly improves cell viability for cell isolation, FACS (fluorescence activated cell sorting) and overall flow cytometry analysis.

The WOLF provides 5 parameters for analyzing and sorting cells. When combined with the N1 Single-Cell Dispenser accessory, it allows higher rates in single cell detection in wells compared to cell printers or limited dilution methods. Deposition above 90-95% are routinely observed.

There is no need to be a flow cytometry expert in order to use the WOLF. The software interface is intuitive, user-friendly and simple. There is no fluidics cart, and the optics are fixed – resulting in less complexity, less clean-up, and more time for experiments.

Compared to a traditional flow cytometry lab setup, the robust, portable, sterile, and safe benchtop system can be used in any lab without the need for dedicated hoods, venting, or personnel. Light enough to be moved and shared. Small enough to have both the WOLF and the N1 single-cell dispenser placed in a biosafety cabinet.

The WOLF is a technology that enables small companies to do things they could not do before.” “The microfluidics capability is something that I have been excited about. The nature of how the WOLF works is being extremely attractive to us. Some of the experiments we’d like to do in the future could not be done on any other device.

Justin Chartron, Ph.D. Protabit LLC

Neurons are extremely delicate so the selection step should be the least disturbing of all if we want to have reliable results. When we tried the WOLF, we were extremely surprised that our cells were very healthy and viability was above 90%, sometimes above 95% which is very impressive.

Dorota Skowronska-Krawczyk, M.Sc.,Ph.D. University of California Irvine

A great machine that has helped us take our cell-engineering pipeline to the next level!

Ronan O'Connell Rice University

The NanoCellect team and technical specialist have been awesome. After two test runs with very involved training/assistance, we have been very pleased with the results. The equipment is intuitive and the sorts have been very clean.

B. Davenport, Ph.D. University of Colorado

NanoCellect Customer Support team has been fantastic in helping us get up and running. The instrument is a helpful tool in our cell line development workflow.

Douglas Austen Takeda Pharmaceuticals

The NanoCellect customer support team is really helpful, knowledgeable and efficient; the WOLF is a great tool in our stem cell research workflow

Helen Miranda, Ph.D. Case Western Reserve University

Applications Overview

The NanoCellect WOLF and WOLF G2 cell sorters are powerful tools used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The WOLF and WOLF G2 are designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The WOLF and WOLF G2 cell sorters are ideal for tasks like cell line development and sorting plant protoplasts,
as well as for monoclonal outgrowth of iPSCs and microalgae.