WOLF cell sorter

WOLF Cell Sorter

  • Healthy Cells

    Live cells can be damaged by conventional cell sorters, resulting in a low-quality cell population. At <2 psi, the WOLF’s microfluidic channel is 30 times gentler than any conventional cell sorter or flow cytometer, allowing for higher populations of healthy, sorted cells via improved cell viability.

  • Contaminant and biohazard free

    Disposable, microfluidic cartridges, and no dangerous aerosol exposure allows for a highly sterile solution and a safer working environment.

  • Easy to use

    Intuitive software, fixed optics, no fluidics cart, less than one minute clean-up time.

  • Flexible

    At under 2 cubic feet, NanoCellect’s new benchmark for access and performance allows every lab the flexibility to do analysis, bulk, or single-cell sorting efficiently..

Redefining Benchtop Cell Sorters

The existing cell sorters in the cell separation market have a common issue of excessive pressure that can compromise cell viability resulting in dead cells. In order to overcome this challenge, NanoCellect has designed an entirely new type of microfluidic cell sorter based on modern flow cytometry methods. By using proven detection technologies, we have been able to focus on innovative cartridge technology (commonly known as a “microfluidic chip”) and accessible, intuitive software that makes the operation simple and safe for researchers and their samples. Our benchtop flow cytometry cell sorter allows for the gentle separation of individual cells and cell types. Sample preparation and sorting cells is easy when using the WOLF in conjunction with our N1 Single Cell Dispenser which utilizes microfluidic channels to transfer and dispense each sorted cell into 96 and 384 multiwell plates.

Flow cytometers and cell isolation products are typically bulky and require a lot of specialized equipment around it. That’s why the WOLF flow cytometry instrument is made compact and stable, enabling placement around the lab and in a hood. The sterile cartridge and tubing set (microfluidic channels) protects target cells during sample preparation from the environment and the researchers from biohazards.

Plus, the software is simple enough to allow new users to perform sorting on day one. Combined with the fact that it’s the most affordable cell sorter on the market, this makes WOLF available at the individual lab level.

Novel Microchip-Based Cell Sorting Technology

Our sorter uses an on-chip piezoacoustic actuator that gently directs cells into collection channels. Our unique system allows cell analysis (cell size and cell type characterization) and sorting to take place within a disposable microfluidic chip. This eliminates sample-to-sample contamination and biohazard aerosols. An embedded cell sorting verification system gives instant feedback of sorting accuracy.

Download WOLF Product Brochure
Novel Microchip-Based Cell Sorting Technology

Take a look inside

488nm Laser and 3 Fluorescent Detectors

The WOLF Cell Sorter uses patented, microfluidic cell sorting and laser-based technology capable of detecting different cell types (mammalian and microbial cells). For FACS (or fluorescence activated cell sorting) a sample of fluorescently labeled cells is focused into a microfluidic flow channel. 

Forward- and Back-Scatter signals are detected by silicon and PMT detectors, respectively. Fluorophores excited by the blue (488 nm) laser beam emit signals that are spectrally filtered to resolve up to 3 colors simultaneously using high-performance PMTs.

Among flow cytometry sorters, WOLF stands shoulders above its competition. Avoid the hassle! If you want a simple cell sort, easy cell sort, and a gentle cell sorter, NanoCellect has your back.

Powerful specs designed to give you an edge

488nm

Powerful and compact
30mW Laser.

Five

Detection parameters: 3 colors, Forward & Back Scatter.

Two

Distinct populations can be sorted at the same time.

24

Microliters per minute of sample can be processed.

Sterile

Prevents the introduction of microorganisms into the sample.

Disposable

Sterile cartridge & fluidic tubing are designed for single day use.

Small Size

14.3H x 15.3W x 15.1D in (36.3 x 38.8 x 38.4 cm)

Sensitive

Can detect unlabeled cells and particles (<1000 MESFs)

Intuitive Software

WOLFViewer

Flow cytometry software

 

The WOLFViewer software has an intuitive workflow menu that walks users through their experimental process. Novice users are guided step-by-step, while expert users can utilize advanced single-cell analysis tools. New users can be performing their first sorts in about 30 minutes and can be on their own on day one. The WOLFViewer:

 

WOLF system
Is easy-to-use and without specialized training
Can be used by both beginners and experts
Moves complex settings to an Advanced Settings dropdown menu to reduce confusion
Can export data in the latest FCS standard file formats for compatibility with other flow software
Allows copy-and-paste of .pdf and .jpg plot images and data to other programs for rapid report and publication generation

When looking for a fragile cell sorting system the WOLF flow cytometry instrument provides intuitive analytics for an overall simple cell sorting solution. Start increasing your sorting efficiency with gentle cell separation and healthier cell populations.