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Explore the power of modern and simple cell sorting.

App Notes

Technical / Workflow

Scientific Posters

High viability cell sorting and 3’ RNA-Seq for gene expression from single cells

Learn how you can combine two technologies: the WOLF® Cell Sorter (NanoCellect) and QIAseq UPX 3’ RNAseq kits (QIAGEN). We provide a complete workflow solution that allows for greater simplicity, improved single-cell RNA-Seq detection, and experimental flexibility.

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Epigenomic Analysis of Sorted Retinal Ganglion Cells using ATAC-seq

Traditionally, Retinal Ganglion Cells (RGCs) have been difficult to sort due to their fragile nature, but the WOLF Cell Sorter has enabled the ability to sort sensitive cell types and prepare a higher quality sample than conventional practices

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Single-Cell Cloning for Cell Line Development

Technologies and techniques for single-cell cloning need a boost. Learn how the WOLF Cell Sorter addresses challenges in sterility, viability and plating efficiency by combining microfluidic cell analysis and sorting with single-cell plating in the cell line development workflow.

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Better Cell Sorting and Antibody Discovery from Single Bovine B-Cells

Bovine immunoglobulins with ultralong CDR H3s have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. However, unlike other species, techniques for studying bovine immunoglobulin genes at the single cell level have not been comprehensively developed. The Snyder Lab has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.

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Gentle Sorting Accelerates Generation of Genome-Edited iPSCs

Low pressure sorting allows for CRISPR-edited induced pluripotent stem cells to be gently plated into a 96-well plate. See how colony outgrowth showed much higher viability compared to other high pressure cell sorters.

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Purification of Multiple Myeloma Plasma Cells with the WOLF Cell Sorter

Multiple Myeloma Plasma cells are a rare and fragile cell type. Learn how the WOLF was able maintain viability and purify a rare cell type from bone marrow cells.

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Increased Viability and Genomic Integrity of CRISPR-modified hiPS cells selected with WOLF® Cell Sorter Microfluidic Technology

Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases, but there are critical shortcomings in the generation of CRISPR-modified clonal lines. See how microfluidic cell sorting can improve overall results compared to a droplet sorter.

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Isolate Antibody-Secreting CHO Cells for Single-Cell Cloning with the WOLF

The therapeutic antibody discovery and development process can last over 10 years before reaching the commercial manufacturing stage. Therefore, it is critical to make every element of this process efficient. Regardless of the initial concentration of target cells, the WOLF accurately detects and sorts antibody-secreting cells with maximum monoclonal outgrowth and titer levels. Whether integrated in-house or combined with the cutting-edge platform of a CMO like JOINN’s, the WOLF’s fluorescence-based cell sorting capabilities, gentle pressure, and sterile workflow make it an essential tool to accelerate the discovery of new therapeutic antibodies.

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Induced Pluripotent Stem Cell Preparation for 10x Genomics Applications

Proper sample preparation is critical for generating high quality sequencing results. Learn how the WOLF improves your sequencing results for 10x Genomics applications and how it compares to conventional cell sorters.

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Microfluidic Sorting for scRNA-Seq using 10x Genomics

Using a gentle cell sorter can improve your sample quality input for 10x Genomics applications. Learn how to the WOLF compares to other cell sorters.

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Gently Sort Digested Skin Tissue Cells on the WOLF

Maintaining a viable single cell suspension from tissue is often challenging due to harsh processing methods. Learn how the gentle WOLF Cell sorter was able to enrich for skin-resident T cells and maintain high viability.

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Plant Nuclei Enrichment and Debris Removal with the WOLF Cell Sorter

Enriching for plant nuclei is challenging due to the large amount of debris in the homogenate. Here, NanoCellect worked with Dr. David Galbraith, a key opinion leader in the plant flow cytometry field to demonstrate that the WOLF® Cell Sorter can be used to effectively and gently remove debris and enrich for nuclei from plant tissue.

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The WOLF Outperforms Traditional Sorters in Isolating Primary Hepatocytes for Evaluation of Ploidy Effect on Proliferation and Organoid Outgrowth

To understand liver physiology and disease development, primary hepatocytes can be isolated and cultured to form 3-dimensional organoids that mimic some in vivo liver behavior. However, isolating primary hepatocytes can be challenging. In this study, the Wang Lab isolated primary hepatocytes with the WOLF and BD FACSAria II to compare instrument performance and evaluate the effect of ploidy on proliferation.

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WOLF® Cell Sorter Purifies Neutrophils with High Viability

Neutrophils have a short half-life and are extremely prone to degradation during processing. Here, the WOLF Cell Sorter’s ability to isolate these delicate cells without impacting viability is demonstrated.

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Detection and Sorting of Microbial Cells and Sub-micron Particles

The WOLF is capable of detecting and sorting small particles. This technical note demonstrates how the WOLF can be used to sort bacteria.

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Seamlessly Transition from Cell Sorter to Single Cell Library Preparation with the WOLF

How you prepare you sample for 10x Genomics applications is key to obtaining optimal sequencing results. The WOLF is able to isolate your target population while removing dead cells, debris and doublets. This technical note demonstrates the sequencing benefits of using the WOLF prior to loading your sample into the Chromium Controller.

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The WOLF Exceeds Limiting Dilution Cell Cloning Efficiency

The N1 Single Cell Dispenser is a valuable tool for generating monoclonal cell lines. Learn how the WOLF performs in comparison to the limiting dilution method.

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Multicolor Cytometry using the WOLF Sorter

Learn how the WOLF Cell Sorter can perform a 2-way sort and compensate 3 colors using FITC, PE and PECy5 labeled beads.

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Single-cell Sorting of T-Cells from a Hetergenous Population of Lyophilized Cells

In collaboration with BioLegend, Veri-CellsTM were used to demonstate the robust capablilties of the WOLF Cell Sorter. Learn how the WOLF Cell Sorter and N1 Single Cell Dispenser can isolate and dispense lyophilized cells from a hetergenous population.

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Gentle WOLF Cell Sorter: T Cell Isolation and Functional Analysis

The WOLF Cell Sorter emits low mechanical stress on cells making it possible to sort fragile cell type. Learn how the WOLF is able to maintain T cell viability and functionality.

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Maximizing Protein Production with WOLF Bulk Sorting

Cell-based protein production systems, in addition to providing commercial lab products such as detection antibodies, are becoming increasingly important for the development and manufacture of biotherapeutics. In efforts to boost protein production, focus is often placed on improving bioreactor systems. However, there is also room for improvement upstream in selecting better, high-producing cells.

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The WOLF “cuts” time from CRISPR workflows

Save time and resources in progressing from hypothesis to fully edited and tested cell lines for challenging academic pursuits or next generation cell therapies. The WOLF Cell Sorter achieves analysis and selection of highly sensitive and fragile cells that would be difficult or impossible to select with traditional cell sorting technologies.

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WOLF Cell Sorter Increases 10x Genomics Library Complexity and Signal-to-Noise

The quality of your sequencing results is directly dependent on sample preparation. This technical note demonstrates how the WOLF can improve your genomic sequencing results.

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Single-cell transcriptomics: combining FACS with plate-based UPX RNA-seq

The most common bottleneck to single-cell analysis is the lack of easy-to-use methods to precisely isolate a single cell from a heterogeneous cell population without damaging the cell and dispensing into 96- or 384-well plates. Learn how combining the power of NanoCellect’s WOLF® Cell Sorter with QIAGEN’s QIAseqTM UPX 3′ Transcriptome kit results in a powerful sample to insight workflow solution.

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Identifying Leukocyte Populations: WOLF Back Scatter vs Traditional Side Scatter

The WOLF operates with a microfluidic flow cell instead of a traditional cuvette flow cell. This requires collecting back scatter, instead of traditional side scatter, to identify cell granularity and complexity. For flow sorting and analysis, it is critical to accurately identify cell type based on granularity, so the WOLF was compared to a traditional cytometer to confirm equivalent performance.

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Techniques for CRISPR/Cas9-Facilitated Genome Editing and Single-Cell Cloning of hPSCs

Low pressure sorting allows for CRISPR-edited induced pluripotent stem cells to be gently plated into a 96-well plate. See how colony outgrowth showed much high viability compared to other high pressure cell sorters.

DOWNLOAD

WATCH WEBINAR

Using human induced pluripotent stem cells and CRISPR/Cas9 genome editing to model autism spectrum disorders

Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases, but there are critical shortcomings in the generation of CRISPR-modified clonal lines. See how microfluidic cell sorting can improve overall results compared to a droplet sorter.

DOWNLOAD

High viability cell sorting and 3’ RNA-Seq for gene expression from single cells

Learn how you can combine two technologies: the WOLF® Cell Sorter (NanoCellect) and QIAseq UPX 3’ RNAseq kits (QIAGEN). We provide a complete workflow solution that allows for greater simplicity, improved single-cell RNA-Seq detection, and experimental flexibility.

DOWNLOAD

Cloning bovine immunoglobulin VH and VL genes from single B cells

Bovine immunoglobulins with ultralong CDR H3s have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. However, unlike other species, techniques for studying bovine immunoglobulin genes at the single cell level have not been comprehensively developed. The Smider Lab has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.

DOWNLOAD

WOLF Cell Sorter for Single-Cell Cloning & Cell Line Development

Technologies and techniques for single-cell cloning need a boost. Learn how the WOLF Cell Sorter addresses challenges in sterility, viability and plating efficiency by combining microfluidic cell analysis and sorting with single-cell plating in the cell line development workflow.

DOWNLOAD