Learn more about how NanoCellect is empowering scientists
Explore the power of modern and simple cell sorting.
Epigenomic Analysis of Sorted Retinal Ganglion Cells using ATAC-seq (App Note)
Department of Ophthalmology, UCSD
Traditionally, Retinal Ganglion Cells (RGCs) have been difficult to sort due to their fragile nature, but the WOLF Cell Sorter has enabled the ability to sort sensitive cell types and prepare a higher quality sample than conventional practices.
Increased Viability and Genomic Integrity of CRISPR-modified hiPS cells selected with WOLF® Cell Sorter Microfluidic Technology (App Note)
Sanford Consortium for Regenerative Medicine, UCSD
Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases, but there are critical shortcomings in the generation of CRISPR-modified clonal lines. See how microfluidic cell sorting can improve overall results compared to a droplet sorter.
Cloning bovine immunoglobulin VH and VL genes from single B cells (Poster accepted at Antibody Engineering & Therapeutics)
Scripps Research, Department of Molecular Medicine and Applied Biomedical Science Institute
Bovine immunoglobulins with ultralong CDR H3s have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. However, unlike other species, techniques for studying bovine immunoglobulin genes at the single cell level have not been comprehensively developed. The Smider Lab has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.
Single-cell RNA-seq analysis: From Cell Sample to Insight
The most common bottleneck to single-cell analysis is the lack of easy-to-use methods to precisely isolate a single cell from a heterogeneous cell population without damaging the cell and dispensing into 96- or 384-well plates. Learn how combining the power of NanoCellect’s WOLF® Cell Sorter with QIAGEN’s QIAseqTM UPX 3′ Transcriptome kit results in a powerful sample to insight workflow solution.
“Cutting” time from CRISPR workflows
Save time and resources in progressing from hypothesis to fully edited and tested cell lines for challenging academic pursuits or next generation cell therapies. The WOLF Cell Sorter achieves analysis and selection of highly sensitive and fragile cells that would be difficult or impossible to select with traditional cell sorting technologies.
Single-cell cloning for cell line development
Technologies and techniques for single-cell cloning need a boost. Learn how the WOLF Cell Sorter addresses challenges in sterility, viability and plating efficiency by combining microfluidic cell analysis and sorting with single-cell plating in the cell line development workflow.
