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Explore the power of modern and simple cell sorting.

App Notes

Technical / Workflow

Scientific Posters

Better Cell Sorting and Antibody Discovery from Single Bovine B-Cells

Bovine immunoglobulins with ultralong CDR H3s have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. However, unlike other species, techniques for studying bovine immunoglobulin genes at the single cell level have not been comprehensively developed. The Smider Lab has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.

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Gentle Sorting Accelerates Generation of Genome-Edited iPSCs

Low pressure sorting allows for CRISPR-edited induced pluripotent stem cells to be gently plated into a 96-well plate. See how colony outgrowth showed much high viablity compared to other high pressure cell sorters.

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High viability cell sorting and 3’ RNA-Seq for gene expression from single cells

Learn how you can combine two technologies: the WOLF® Cell Sorter (NanoCellect) and QIAseq UPX 3’ RNAseq kits (QIAGEN). We provide a complete workflow solution that allows for greater simplicity, improved single-cell RNA-Seq detection, and experimental flexibility.

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WATCH WEBINAR

Purification of Multiple Myeloma Plasma Cells with the WOLF Cell Sorter

Multiple Myeloma Plasma cells are a rare and fragile cell type. Learn how the WOLF was able maintain viablity and purify a rare cell type from bone marrow cells.

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Increased Viability and Genomic Integrity of CRISPR-modified hiPS cells selected with WOLF® Cell Sorter Microfluidic Technology

Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases, but there are critical shortcomings in the generation of CRISPR-modified clonal lines. See how microfluidic cell sorting can improve overall results compared to a droplet sorter.

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Epigenomic Analysis of Sorted Retinal Ganglion Cells using ATAC-seq

Traditionally, Retinal Ganglion Cells (RGCs) have been difficult to sort due to their fragile nature, but the WOLF Cell Sorter has enabled the ability to sort sensitive cell types and prepare a higher quality sample than conventional practices

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Single-Cell Cloning for Cell Line Development

Technologies and techniques for single-cell cloning need a boost. Learn how the WOLF Cell Sorter addresses challenges in sterility, viability and plating efficiency by combining microfluidic cell analysis and sorting with single-cell plating in the cell line development workflow.

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Multicolor Cytometry using the WOLF Sorter

Learn how the WOLF Cell Sorter can perform a 2-way sort and compensate 3 colors using FITC, PE and PECy5 labeled beads.

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Single-cell Sorting of T-Cells from a Hetergenous Population of Lyophilized Cells

In collaboration with BioLegend, Veri-CellsTM were used to demonstate the robust capablilties of the WOLF Cell Sorter. Learn how the WOLF Cell Sorter and N1 Single Cell Dispenser can isolate and dispense lyophilized cells from a hetergenous population.

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T Cell Isolation and Downstream Applications using the WOLF Cell Sorter

The WOLF Cell Sorter emits low mechanical stress on cells making it possible to sort fragile cell type. Learn how the WOLF is able to maintain T cell viablity and functionality.

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Maximizing Protein Production with WOLF Bulk Sorting

Cell-based protein production systems, in addition to providing commercial lab products such as detection antibodies, are becoming increasingly important for the development and manufacture of biotherapeutics1. In efforts to boost protein production, focus is often placed on improving bioreactor systems. However, there is also room for improvement upstream in selecting better, high-producing cells.

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Seamlessly Transition from Cell Sorter to Single Cell Library Preparation with the WOLF

How you prepare you sample for 10x Genomics applications is key to obtaining optimal sequencing results. The WOLF is able to isolate your target population while removing dead cells, debris and doublets. This technical note demonstrates the sequencing benefits of using the WOLF prior to loading your sample into the Chromium Controller.

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The WOLF Exceeds Limiting Dilution Cell Cloning Efficiency

The N1 Single Cell Dispenser is a valuable tool for generating monoclonal cell lines. Learn how the WOLF performs in comparison to the limiting dilution method.

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The WOLF “cuts” time from CRISPR workflows

Save time and resources in progressing from hypothesis to fully edited and tested cell lines for challenging academic pursuits or next generation cell therapies. The WOLF Cell Sorter achieves analysis and selection of highly sensitive and fragile cells that would be difficult or impossible to select with traditional cell sorting technologies.

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WOLF Cell Sorter Increases 10x Genomics Library Complexity and Signal-to-Noise

The quality of your sequencing results is directly dependent on sample preparation. This technical note demostrates how the WOLF can improve your genomic sequencing results.

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Single-cell transcriptomics: combining FACS with plate-based UPX RNA-seq

The most common bottleneck to single-cell analysis is the lack of easy-to-use methods to precisely isolate a single cell from a heterogeneous cell population without damaging the cell and dispensing into 96- or 384-well plates. Learn how combining the power of NanoCellect’s WOLF® Cell Sorter with QIAGEN’s QIAseqTM UPX 3′ Transcriptome kit results in a powerful sample to insight workflow solution.

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Detection and Sorting of Microbial Cells and Sub-micron Particles

The WOLF is capable of detecting and sorting small particles. This technical notes demonstrates how the WOLF can be used to sort bacteria.

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WOLF Cell Sorter Preserves Neutrophil Viability and Activation Levels

Neutrophils have a short half-life and are extremely sensitive to activation during processing. Here, the WOLF Cell Sorter’s ability to isolate these delicate cells without impacting viability and activation is demonstrated.

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Using human induced pluripotent stem cells and CRISPR/Cas9 genome editing to model autism spectrum disorders

Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases, but there are critical shortcomings in the generation of CRISPR-modified clonal lines. See how microfluidic cell sorting can improve overall results compared to a droplet sorter.

DOWNLOAD

High viability cell sorting and 3’ RNA-Seq for gene expression from single cells

Learn how you can combine two technologies: the WOLF® Cell Sorter (NanoCellect) and QIAseq UPX 3’ RNAseq kits (QIAGEN). We provide a complete workflow solution that allows for greater simplicity, improved single-cell RNA-Seq detection, and experimental flexibility.

DOWNLOAD

Techniques for CRISPR/Cas9-Facilitated Genome Editing and Single-Cell Cloning of hPSCs

Low pressure sorting allows for CRISPR-edited induced pluripotent stem cells to be gently plated into a 96-well plate. See how colony outgrowth showed much high viablity compared to other high pressure cell sorters.

DOWNLOAD

Cloning bovine immunoglobulin VH and VL genes from single B cells

Bovine immunoglobulins with ultralong CDR H3s have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. However, unlike other species, techniques for studying bovine immunoglobulin genes at the single cell level have not been comprehensively developed. The Smider Lab has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.

DOWNLOAD

WOLF Cell Sorter for Single-Cell Cloning & Cell Line Development

Technologies and techniques for single-cell cloning need a boost. Learn how the WOLF Cell Sorter addresses challenges in sterility, viability and plating efficiency by combining microfluidic cell analysis and sorting with single-cell plating in the cell line development workflow.

DOWNLOAD