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Discover the World's First Two-Laser
Image-guided Cell Sorter

The VERLO Image-Guided Cell Sorter expands upon NanoCellect’s gentle microfluidic-based cell sorting technology to enable researchers to visualize, analyze and sort cells based off image features and cell morphology.

With two lasers and nine colors, plus 3 label-free parameters and a total of 12 imaging channels, VERLO introduces significant advances in imaging flow cytometry analysis and cell sorting.

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Gentle Cell Sorting with the Most
Image-Based Parameters

Enable cutting-edge workflows for cell analysis and cell sorting

The VERLO combines traditional flow cytometry with image-based technology.

Sterile cell sorting

Microfluidic cartridge provides complete interchangeable fluidics system to prevent cross-contamination and eliminate aerosols - keeping you and your samples safe.

Experience the most image-based flexibility

With two imaging lasers, nine fluorescent and three label-free channels, the VERLO allows more flexibility for assay design.

Enhanced cell viability post sort with flexible media capabilities

NanoCellect’s microfluidic cartridge utilizes gentle sorting mechanism and low pressure (<2 psi) to keep cells happy and healthy. Disposable nature of cartridge enables greater flexibility in media choice.

Compatible machine learning tools

Enhanced novel label-free and fluorescent gating strategy with 3rd party machine learning tools to identify new cell populations.

Best-In-Class Clonal Outgrowth

The VERLO is integrated with 96- and 384-well dispensing and its gentle microfluidics yielding in higher number of clones per plate.

Explore 3 Label-Free Channels and
9 Fluorescent Channels

Image Acquisition

Cell detection scanning 2 modulated collinear lasers

Waveform signals from all channels are used to reconstruct label-free and fluorescent images prior to analysis and image features extraction

VERLO Image-Guided Cell Sorter Image Formation

Features Extraction

Images are analyzed and image features are extracted for plotting and defining a gating strategy

Discover the VERLO™

This presentation introduces the VERLO™ Image-Guided Cell Sorter from NanoCellect. VERLO integrates advanced image-guidance technology with cell sorting, enhancing precision and efficiency.

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Applications

Sort based on Cell-Cell Interactions

Detect and preserve cellular associations throughout an entire sort

Sort based on Intracellular Location

Visualize and sort based on intracellular protein dynamics

Sort based on fluorescence protein expression

With a 488nm and a 561nm laser, the VERLO can sort live cells expression multiple fluorescent proteins

CellVantage Software

CellVantage software combines classic flow cytometry features with an interactive image gallery to visualize all analyzed and sorted cells.

• Intuitive workflows for instrument set-up and analysis.
• Customizable image gallery for cell population visualization.
• Index sorting.
• FCS 3.0 or ICE file exports.
• Automatic and manual compensation.

Software and integrated features

Gentle Microfluidic Technology

The VERLO™ Image-Guided Cell Sorter uses patented, microfluidic-based sorting to isolate mammalian cells, microbes, plant cells and more. A gentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format.

• Disposable cartridges that allow for bulk sorting or single-cell sorting.
• Sort up to 200 cells per second with high accuracy and effective recovery.
• Sort two selected cell populations with bulk sorting and no waste of unsorted cells.
• Deposit 1 to 100 cells per well in a 96- or 384 well.

Integrated Single-Cell Dispensing and Temperature Control

Designed to sort and dispense into 96- and 384-well plates, the integrated dispenser provides higher rates of singlet detection compared to cell printers or limiting dilution.

• Bulk sorting in 5 mL and 50 mL plates
• 96-well plating: 6 min
• 384-well plating: 20 min
• Dispensed Volume in plates: 4 µL
• Plate options: 96-or 384-well plates (flat / U-bottom / V-bottom or PCR)

The VERLO has integrated independent sample cooling and optional sample agitation to maintain them in uniform suspension.

• Chilling Temperature: 4-24°C (39.2-75.2°F)
• Sample Rotation Speed: 5-100 rpm