ShopSort Cells You Can See With the Gentle Microfluidics You Know
Discover the World's First Two-Laser
Image-guided Cell Sorter
The VERLO™ Image-Guided Cell Sorter is providing conventional flow cytometry and cell sorting along with the ability to perform imaging analysis to identify and sort cells based on morphology, subcellular localization and more.
The VERLO™ instrument expands the capabilities of gentle benchtop microfluidic cell sorting. With two lasers and nine colors, plus 3 label-free parameters, it maintains simple workflows for either bulk sorting or single-cell dispensing into 96- or 384-well plates with an integrated cell dispensing and sample cooling.
The VERLO™ instrument expands the capabilities of gentle benchtop microfluidic cell sorting. With two lasers and nine colors, plus 3 label-free parameters, it maintains simple workflows for either bulk sorting or single-cell dispensing into 96- or 384-well plates with an integrated cell dispensing and sample cooling.
This presentation introduces the VERLO™ Image-Guided Cell Sorter from NanoCellect. VERLO integrates advanced image-guidance technology with cell sorting, enhancing precision and efficiency. The system captures images of individual cells, providing spatial information such as cell morphology and marker localization. Enhanced resolution imaging capabilities allow for the visualization and analysis of subcellular structures and spatial variations in brightfield, darkfield, and fluorescent images. VERLO’s gating strategy incorporates imaging features to define cell phenotypes, increasing the precision of cell sorting. The system combines low-pressure microfluidics with single-cell image acquisition to measure a multitude of features from cell images, generating comprehensive multivariate datasets.
Adding Image Analysis to
Gentle Microfluidic Cell Sorting
Key VERLO™ Applications
VERLO™ Image Sorting Technology
12-Parameter Image-Guided Sorting
With 488 nm and 561 nm lasers to detect up to 9 fluorescent markers and 3 channels of label-free information, the VERLO Image-Guided Cell Sorter can identify key structures, such as golgi and nuclei, internalization, overall morphology, and nuanced texture features that can discriminate live-dead—as well as the physiology of the cells.
Once you have decided on which morphological features of the cells you want to sort, you can gate on image features such as Area vs Max Intensity, just like you would when sorting using FSC vs BSC. The image features are calculated in real-time, enabling you to begin sorting your samples without having to first analyze the whole sample and then run it through the instrument again.
Microfluidics
The VERLO™ Image-Guided Cell Sorter uses patented, microfluidic-based sorting with robust laser-excitation and sensitive PMT detectors to isolate mammalian cells, microbes, plant cells and more. A gentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup. This technology allows the VERLO™ to sort up to 200 cells per second with high accuracy and effective recovery. Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser. CellVantage Software VERLO™ integrated platform uses patented, microfluidic-based sorting with robust laser-excitation and sensitivePMT detectors to isolate mammalian cells, microbes, plant cells and more. Agentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup.
- Unique to NanoCellect are our disposable cartridges that allow for bulk sorting or single-cell sorting.
- The sorting cartridges use a piezoacoustic actuator that gently directs cells into collection channels; an embedded cell sorting verification system gives instant feedback of sorting accuracy.
- This technology allows the VERLO™ to sort up to 200 cells per second with high accuracy and effective recovery.
- Sort two selected cell populations with bulk sorting while the remainder of cells collects in a third channel.
- Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser.
VERLO™ Specifications
| Sample input | 1.5, 2.0, and 5.0 mL tubes |
| Sheath input | 50 mL conical tubes |
| Sheath fluid | Buffer of choice |
| Sheath fluid usage | 9.6 mL/hour |
| Sample flow rate | 12 μL/minute |
| Sample line volume | 50 μL |
| Minimum sample volume | 100 μL |
| Tubing diameter (inner) | 200 to 500 μm |
| Flow cell | 200 x 700 μm |
| Smaller channel diameter | 70 μm |
| Sample pressure | < 2 psi |
| Sample output (bulk sorting) | 1.5 mL or 5 mL tubes |
| Sample output (single cell) | 96- or 384-well plates (flat/U/V bottom or PCR) |
| Time to plate (96 wells) | 6 minutes |
| Time to plate (384 wells) | 20 minutes |
| Droplet volume | 4 μL |
| Sample plate options | 96 or 34 well (flat bottom, V-bottom, U-bottom, PCR) |
| Scatter sensitivity | < 1.5 μm by FSC or BSC |
| Scatter resolution | Resolves lymphocytes, monocytes and granulocytes |
| Fluorescence sensitivity | < 200 MESF FITC (488 nm laser)< 250 MESF PE (561 nm laser) |
| Fluorescence resolution | 9-peak separation with SPHERO Rainbow Calibration Particles |
| Analysis speed | 2,000 events/second |
| Sorting | 1- and 2-ways |
| Back-to-back sorting speed | 200 events/second |
| Absolute counts | Yes |
| Volumetric counts | Yes |
| Warm-up time | Less than 1 minute |
| Sorting purity | Up to 99% purity |
| Laser Profile | 1.5 x 1.5 μm |
| Scatter detection | Forward (0 degrees, +/- 15) Back (180 degrees, +/- 15) |
| Excitation & emission detection | 488 mm 50 mW: 525/50, 580/25, 620/50, 706/95, 760LP 561nm 50 mW: 580/25, 620/50, 706/95, 760LP |
| Optical alignment | Fixed alignment No maintenance required |
| VERLO Dimensions | 18 in H x 40 in W x 18 in D (45.7 cm H x 101.6 cm W x 45.7 cm D) |
| VERLO weight | 150 lbs / 68 kg |
| VERLO Electrical | AC Input: 100-240V, 50-60Hz, 2A |
VERLO™ Configuration
The VERLO’s dual laser configuration allows 9 fluorescent and 3 label-free flow cytometry and imaging channels to be collected.
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The WOLF G2 is an invaluable tool for Stem Cell Research
The WOLF G2 also enables researchers to sort and dispense individual stem cells directly into well plates, a process essential for clonal isolation and downstream analyses. With its user-friendly design and exceptional reliability, the WOLF G2 simplifies the complex task of stem cell sorting, making it an indispensable asset for advancing stem cell research.
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VERLO™ Integrated Platform
VERLO's integrated system offers seamless compatibility and streamlined workflows, providing researchers with a comprehensive solution for their cell sorting needs.
CellVantage™ Software
The CellVantage software combines the classic flow cytometry features familiar to flow cytometry scientists with an interactive image gallery to visualize all analyzed and sorted cells. The software has an intuitive workflow interface that walks the user through the experimental process and is designed for both novice and expert users.
Single-Cell Dispenser
Designed to sort and dispense into 96- and 384-well plates, the integrated dispenser provides higher rates of singlet detection comparted to cell printers or limiting dilution. Users can preform simple, label-free dispensing or advanced multicolor panel single-cell dispensing.
Plate sorting specifications
Time to Plate (96-well): 6 min
Time to Plate (384-well): 20 min
Dispensed Volume: 4 µL
Plating options: 96-or 384-well plates
(flat/U/V bottom or PCR)
Sample Cooling and Mixing
The VERLO™ has integrated sample cooling designed to keep your temperature-sensitive samples cooled and agitated to maintain them in uniform suspension while running bulk and single-cell sorting. Both your sample and sheath fluid are kept at the desired temperature. In addition, sorted cells are also kept cooled whether you sort in tube or multi-well plates.
Sample cooling and mixing specifications
Chilling Temperature: 4-24°C (39.2-75.2°F)
Sample Rotation Speed: 5-100 rpm
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