The WOLF G2® Cell Sorter uses patented, microfluidic-based sorting with robust laser-excitation and sensitive PMT detectors to isolate mammalian cells, microbes, plant cells and more. A gentle and piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup. This technology allows the WOLF G2 to sort up to 200 cells per second with high accuracy and effective recovery. Deposit 1 to 100 cells per well in a 96-well or 384-well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser.

• Unique to NanoCellect are our disposable cartridges that allow for bulk sorting or single-cell sorting.
• The sorting cartridges use a piezoacoustic actuator that gently directs cells into collection channels; an embedded cell sorting verification system gives instant feedback of sorting accuracy.
• Sort two selected cell populations with bulk sorting while the remainder of cells collects in a third channel.
• Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser.

The WOLF G2® Cell Sorter is an advanced benchtop microfluidic cell sorter designed for gentle microfluidic cell sorting. It features dual laser capabilities and supports up to nine fluorescent colors, providing researchers with robust options for complex experimental designs. The WOLF G2 maintains sterility and low-pressure sorting, ensuring the integrity and viability of delicate cells, such as stem cells and primary cells. The instrument's intuitive workflow supports both bulk sorting and single-cell dispensing into 96- or 384-well plates when used with the N1 Single-Cell Dispenser, making it a versatile tool for applications ranging from single-cell genomics and cell line development to gene editing and antibody discovery.

Designed to sort and dispense into 96- and 384- well plates, the N1 provides higher rates of singlet detection compared to cell printers or limiting dilution. Users can perform simple, label-free dispensing or advanced multicolor panel single-cell dispensing.

Plate sorting specifications
Time to plate (96 wells): 3 - 8 minutes
Time to plate (384 wells): 32 minutes
Droplet volume: 3 - 10 µL
Sample plate options: 96 or 384 wells (flat bottom, V-bottom, U-bottom, PCR)

Applications with temperature sensitive samples, such as Stem cells or Neuronal cells can be challenging to sort. Such applications would benefit from modules such as the CS1 Chiller-Stirrer by keeping both sheath fluid and sample at the desired temperature during the sorting process.

The WOLFViewer software has an intuitive workflow menu that walks users through their experimental process, and is designed for both novice and expert users. New users can be performing their first sorts in about 20 minutes and can be on their own on day one.

Plate sorting specifications
System power up: 3 minutes
System setup and calibration: 20 minutes
System shutdown: 3 minutes
Additional features: Auto-alignment, intuitive compensation, advanced coloring and gating options, FSC files compatible with FlowJo