Simplified sample prep and higher quality results.
Single Cell Genomics
Our understanding of science is constantly changing. Scientists across the globe are constantly finding new ways to pioneer emerging fields and they require cutting edge tools and devices to do so. Our goal at NanoCellect was to create a benchtop flow cytometry, cell sorting and dispensing system for these researchers to leverage in their scientific journeys.
The scientific study of genomics has progressed immensely over the last decade. While there are many approaches to tackling modern day medical issues and genomic
analysis, single cell genomics allows for a more narrowed focus and study of a specific cell where the data doesn’t come from a cell population average. Instead, the data from each of the individual cells is measured. Prior to the growth of single cell genomics, the issue had been isolating the single cells of interest and easy amplification of the sample for study. In order to fully understand specific cell types and how they interact on a cellular level, we must analyze the RNA & DNA sequencing data of each of these cells on an individual level. For example, this is useful for studying gene mutations (within tumor cells) and gene expression. With technological innovations in the single cell field, it’s now easier than ever to prepare single cell samples with our benchtop cell sorting system and dispenser.
Sample preparation is a critical (yet often underappreciated) step in single cell genomics, single-cell analysis for various fields of research. Many current methods of sample prep can risk damaging its cellular structure; however, NanoCellect’s microfluidic process allows for cell suspension in fluid to protect the cell’s integrity for the purposes of DNA & RNA single cell sequencing. When it comes to improvements in cell sample purity and viability, the following areas can benefit greatly:
- Next generation sequencing on Illumina or IonTorrent platforms
- Genomic library prep with 10X Genomics or Fluidigm systems
- Plate based RNA-seq kits from QIAGEN or Takara
Optimizing your single cell preparation can maximize your cell samples in two ways:
- By inputting the “right” cells with selection based on viability and other parameters if needed
- By utilizing sorting technology that leaves cells or nuclei intact.
That’s where the WOLF Cell Sorter comes in.
By selecting and enriching only the target cells of interest you can avoid the need to sequence and analyze dying cells, debris, and non-target cells—directly impacting your total cost per cell analyzed and allow researchers more time to plan experiments efficiently.
The NanoCellect WOLF Cell Sorter also provides accurate cell count of selected cells and eliminates the need for a cell counter or viability check prior to your analytic step. In addition, the WOLF provides an easy-to-use cell enrichment device and single cell dispenser that does not require expertise in flow cytometry. NanoCellect’s system is:
- Robust, portable, and sterile
- Safe for single cell DNA and RNA sequencing
- Efficient in single cell sorting and cloning operations
- Can be used in any lab without the need for dedicated hoods, venting, or expert personnel
The sterile, exchangeable fluidics system of the WOLF eliminates carry-over between samples and makes clean up a snap. When in doubt about how to optimize efficiencies in your lab, just ask yourself:
What is single cell sorting without WOLF? Cumbersome.
What is single cell sorting with WOLF? Ideal.
That’s the NanoCellect guarantee—that’s happy cells.