Avoid karyotype issues with gentle microfluidic sorting.

gene editing workflow

Gene editing, such as the use of CRISPR technology, is inherently a complicated and sensitive process. With the complexity of the correct modification, identification and clonal expansion of a single cell, flow cytometry and sorting are useful, but often avoided for a variety of reasons. Although conventional cell sorters rapidly sort high volumes, they induce a great deal of shear stress on cells, diminishing their integrity and leaving researchers with a high population of unusable cells. Additionally, they typically require an expertise in flow cytometry to operate, involve a cumbersome fluidics carts and preparation protocols.

The WOLF Cell Sorter and N1 Single Cell Dispenser were developed to help ease the workflow, and ensure a higher population of healthy cells.

The WOLF’s gentle, sterile microfluidics enable scientists to

  • work faster and deliver better results to your customer
  • get to genomic discovery faster
  • potentially speed up your time to publication

High cell integrity can be your new normal. With a sorting pressure of <2 psi, the WOLF is 30 times more gentle than standard conventional sorters, and 10 times more gentle than even the most gentle conventional sorter available today. Additionally, you can use growth media as sheath so that the cells are always in their most optimal environment. Cloning from single, gene-edited cells is super easy and works with both 96 or 384-well plates.

Easy to use, the WOLF can improve your workflow efficiency, reducing your hours spent and enabling faster time to answer. With intuitive software, fixed optics and a sterile, rapidly exchangeable cartridge, you do not need to be an expert in flow cytometry to operate the instrument.

Visit the Scientific Content section of our web site to read how researchers at the Sanford Consortium for Regenerative Medicine are experiencing Increased Viability and Genomic Integrity of CRISPR-modified hiPS cells selected with WOLF Cell Sorter Microfluidic Technology.