Improve your discovery workflow.

antibody discovery workflow

We understand the workflow of antibody discovery is not without its challenges. With the complexities of  variations on immunization, isolation of B-cells from blood, plating of single viable cells, and sequencing of cells, we designed the WOLF Cell Sorter to enhance key steps in this workflow. First, by sorting B-cells from blood, the relevant cells can be collected for downstream characterization and plating. Once reactive B-cells are identified, gentle, sterile sorting and single-cell plating into 96- and 384-well plates for expansion, further characterization, and sequencing can be performed in the lab.

The WOLF provides easy-to-use cell enrichment and single-cell dispensing without the need for flow cytometry expertise. The robust, portable, sterile, and safe system can be used in any lab without the need for dedicated hoods, venting, or personnel.

The rapidly-exchangeable cartridge and tubing set can easily set-up and taken down in minutes to eliminate sample carry over and time consuming cleaning protocols.

Visit the Scientific Content section of our web site to read how researchers at The Smider Lab at Scripps Research has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.