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cell-line-development workflow

Say good-bye to the frustrations of limiting dilution and the shear stress caused by conventional flow cytometers. Contaminate and biohazardfree, the WOLF Cell Sorter’s rapidly-exchangeable cartridge and tubing set eliminates carryover between samples and allows for quick and easy clean up.

When paired with the N1 Single Cell Dispenser, the WOLF can be used to select viable single cells and plate them into 96 or 384well plates. The wells can be pre-loaded with culture media, and the same media can be used as the sheath buffer. This, in conjunction with the low pressure induced by the sorter, greatly improves cell integrity from that of conventional sorters, providing higher populations of viable, healthy cells, facilitating stable cell line development.

How gentle is the WOLF? With <2 psi systematic pressure, the WOLF is 30 times gentler than standard cell sorters. It is so easy to use that virtually anybody can be an expert in cell line engineering.

More than a cell printer, the WOLF can sort based on 5 optical parameters and provide well-by-well indexed cellular data.

As an example, consider your lab tech is working on CHO cell line development. CHO-S cells (and, frankly, anything “CHO”) are the gold standard for manufacturing therapeutic proteins. However, with traditional cytometers, the cell culture process development is rendered inefficient by how much cell death takes place during the cell sorting. The same holds for:

  • iPSC cell line development (and iPSC reprogramming)
  • Immunophenotyping efforts
  • Cancer research and drug testing

Using NanoCellect products, cell line development and engineering has never been safer for cell populations and easier for lab techs. With easy-to-use hardware for testing and intuitive software for analytics, the WOLF cell sorter is your platform of choice for simple cell sorting.

Think NanoCellect for a happier, healthier lab.

Visit the Scientific Content section of our web site to read how gentle, single-cell sorting with the WOLF and N1 can help improve the cell line development process.