Pairing Uniform Whole-Genome Amplification with Simple Single-Cell Sorting

Pairing Uniform Whole-Genome Amplification with Simple Single-Cell Sorting

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Introduction

Whole-genome sequencing of single cells involves a multitude of technical challenges:

  • Physically isolating single cells
  • Obtaining sufficient genomic material for downstream analyses
  • Low and variable genomic coverage
  • Amplification artifacts such as allelic bias, mutations, and chimeras

However, NanoCellect and BioSkryb have established a workflow to simplify this process and bypass these hurdles. This workflow profile highlights how next-generation cell sorting and sequencing technologies can be used to obtain high quality single-cell genomic data.

Upstream, NanoCellect’s WOLF Cell Sorter:

  •  Gently and accurately isolates single cells
  • Confidently avoids contamination concerns
  • Easily integrates into any workflow with a small footprint <2 ft3 and a user-friendly interface
  • Affordably fits any budget

Downstream, BioSkryb’s ResolveDNA™ platform:

  • Directly copies single-cell genomes or low-input DNA with Primary Template-direct Amplification1
  • Amplifies with unprecedented genomic coverage uniformity and breadth
  • Precisely thwarts error propagation with high allelic balance
  • Collectively, enables hitherto unachieved confidence in single nucleotide variant (SNV) and copy number variation (CNV) calling from single cells

 

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