Genetic engineering methods that use viral, electroporation, lipofection or nanoparticles to introduce CRISPR, transposon, and other approaches are imperfect and require downstream selection of the edited cells. The WOLF G2 and N1 Single-Cell Dispenser lead the pack with gentle, sterile, and easy-to-use microfluidic technology to carry edited cells forward for monoclonal expansion and karyotyping. Bring the sorter to your cells by placing the WOLF and N1 on your bench or in your biosafety cabinet and sort on your cells’ schedule.

The strength of iPSC models has been confirmed by recent FDA approvals of drugs to begin clinical trials based on iPSC-derived organoid data—replacing animal models. But reprogramming fibroblasts to iPSCs, genetically engineering the cells, and driving pluripotent cells to specific lineages can be an inefficient processes. The WOLF G2 Cell Sorter and N1 Single-Cell Dispenser differentiate themselves in single-cell cloning performance. The WOLF preserves pluripotency with gentle, low-pressure, and sterile microfluidic sorting. The N1 rapidly dispenses single cells into 96- or 384-well plates.