Botanical Brilliance
Mastering the Art of Plant Cell Sorting
Sorting plant cells poses unique challenges due to their structural complexity and diverse characteristics. The rigid cell walls of plants require specialized techniques to effectively isolate and sort cells without compromising their integrity. Additionally, plant cells often vary significantly in size, shape, and composition, making it challenging to achieve uniform sorting outcomes.
The WOLF G2 Cell Sorter offers a method to isolate and enrich for plant cells and organelle populations to improve omics discoveries, and enrich gene-edited protoplasts or embryogenic microspores for accelerated double haploid production and plant regeneration. With the WOLF G2, you can sort viable fragile cells with high viability.
Maintain Optimal Plant Cell Health and Regeneration Property
Sort Protoplasts in Custom Osmolarity Media
Sorting plant protoplasts is challenging with conventional cell sorters due to high sample pressures, shear stress and osmotic change which damage cells making them unusable for downstream applications. The ability of the WOLF G2 to enrich and sort protoplasts is essential to haploid plants regeneration.
Protoplast isolation and enrichment using the microfluidic WOLF® Cell Sorter
Tab Content
The WOLF G2 is an invaluable tool for Stem Cell Research
Tab Content
Tab Content
Isolate Microspores for Haploid Regeneration
Microspores are crucial in plant breeding programs and genetic studies, but their delicate nature requires specialized techniques for isolation and sorting. The WOLF G2 offers a solution with its gentle microfluidic technology, allowing for precise and efficient sorting of microspores while maintaining their viability and integrity. This enables researchers to obtain pure and homogeneous populations of microspores, facilitating downstream analyses and advancing research in plant genetics and breeding.
cell size (FSC-H), internal complexity/granularity (BSC-H), and green autofluorescence (FL1, B525-H) all excited by the 488nm laser. (Left) Subsequent child gate selecting for green auto fluorescent positive events (gated to singlets). (Right) a histogram representation of the green positive events within the child gate “singlets.”
Regeneration of Plant Embryogenic Microspores Enriched with the WOLF G2® Gentle Cell Sorter
Tab Content
The WOLF G2 is an invaluable tool for Stem Cell Research
Tab Content
Tab Content
Sort Plant Nuclei for Dowstream Genomics
Sorting nuclei on the WOLF G2 is an important part of omics workflows and lower-throughput ploidy analyses. The WOLF G2 excels in preserving nuclei integrity throughout the sorting process, ensuring improved sequencing reads and accurate ploidy results. Unlike traditional cell sorters, the WOLF G2's gentle microfluidic technology delicately handles nuclei, minimizing cellular stress and maximizing the quality of sorted samples.
Plant Nuclei Enrichment and Debris Removal with the WOLF Cell Sorter
Gentle enrichment of plant protoplasts and nuclei using a microfluidic cell sorter
Tab Content
The WOLF G2 is an invaluable tool for Stem Cell Research
Tab Content
Isolate Microalgae for Environmental Studies
Sorting microalgae presents unique challenges due to their small size and delicate nature. The WOLF G2, equipped with microfluidic technology, offers a solution by providing gentle sorting of microalgae. This instrument allows researchers to efficiently isolate and purify microalgal cells, ensuring high viability and integrity post-sorting. With the WOLF G2, scientists can overcome the complexities of sorting microalgae and advance research in areas such as biofuel production, bioremediation, and bioprospecting for novel compounds.
Enrichment and growth of transgenic microalgae using a gentle microfluidic cell sorter
Tab Content
The WOLF G2 is an invaluable tool for Stem Cell Research