Sample preparation is a significant yet underappreciated step that allows for high quality sequencing and analysis, critical to achieving breakthroughs in medicine, agriculture, and other industries. NanoCellect’s microfluidic cell sorter, the WOLF, allow the cell’s integrity to be maintained for numerous sequencing and multi-omics techniques. The WOLF’s ability to flexibly fit into any genomic researcher’s lab, make it a key step to any cell sequencing analysis workflow.

• 10x Genomics RNA-seq library prep
• Single-Cell sorting on PCR plate
• Whole-genome sequencing methods

The antibody drug discovery process differs from the traditional drug discovery of small molecules as it requires the development of hybridomas and the engineering of antibodies to recognize a specific target protein. The production of recombinant antibodies in cancer treatment as well as the development of neutralizing antibodies for infectious diseases, has allowed antibody discovery platforms to interrogate the immune repertoire and develop novel antibody libraries using flow cytometry analysis and cell sorting. The WOLF cell sorter provides a simple, gently cell sorting solution for many workflows such as:

• Antibody Discovery from Single Bovine B-Cells
• Isolate Antibody-Secreting CHO Cells
• Recombinant protein production in CHO Cells

Generating cell lines has often been inefficient and affected by inaccurate limited dilution methods or the shear stress caused by conventional flow cytometer and traditional high-pressure cell sorters. NanoCellect Biomedical provides cell sorting solutions that allow to sort cells and establish stable cell lines using gentle low-pressure microfluidics. The WOLF’s ability to protect cell viability after sorting makes it an integral part of any cell line development workflow such as:

• Recombinant protein production in CHO Cells
• Sorting stem cell cultures
• Antibody discovery

Isolating edited-pluripotent stem cells with traditional sorting techniques can subject the cells to rough conditions in which gene expression, genomic stability and overall cell-health can become compromised. Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. Learn more about how cell sorting can improve workflows such as:

• CRISPR and disease modelling
• Protection of Karyotype
• Generation of Genome-Edited iPSCs