Botanical Brilliance Mastering the Art of Plant Cell Sorting
Sorting plant cells poses unique challenges due to their structural complexity and diverse characteristics. The rigid cell walls of plants require specialized techniques to effectively isolate and sort cells without compromising their integrity. Additionally, plant cells often vary significantly in size, shape, and composition, making it challenging to achieve uniform sorting outcomes.
The WOLF G2 Cell Sorter offers a method to isolate and enrich for plant cells and organelle populations to improve omics discoveries, and enrich gene-edited protoplasts or embryogenic microspores for accelerated double haploid production and plant regeneration. With the WOLF G2, you can sort viable fragile cells with high viability.
What Customers Say
Read firsthand accounts of how our cell sorting solutions are transforming scientific discoveries.
Right now, the best technology out there for [single stem cell cloning] is the WOLF.
Pluristyx
Single cell clonal outgrowth is “hovering at 45% with the WOLF, whereas with [a droplet based cell sorter] we maxed out at about 35%".
Large Pharma Scientist
The WOLF is easier for a non-crazy engineer to understand and use.
Mt. Sinai
Maintain Optimal Plant Cell Health and Regeneration Property
Keep Your Protoplasts Intact
Minimize cellular stress during sorting procedures with the WOLF G2's low-pressure microfluidic technology, ensuring the integrity of sensitive plant cell samples.
Ensure Plant Cell Viability
The gentle sorting mechanism of the WOLF G2 preserves the viability of delicate plant cells, ensuring optimal health and functionality post-sorting.
Sort on Chlorophyll Content
Achieve high purity levels in sorted plant cell samples, minimizing contamination and enhancing the reliability of downstream analyses.
Use Custom Plant Media
Ability to use plant cell culture medium as sheath and sample media. WOLF G2 is compatible with a range of osmolarity.
Microspores for Plant Breeding
Obtain pure and homogeneous populations of microspores, facilitating downstream analyses and advancing research in plant genetics and breeding.
Small and Mighty
No bulky fluidic cart or lines brings cell sorting right to your benchtop, boat, submarine or wherever science takes you.
Sort Protoplasts in Custom Osmolarity Media
Sorting plant protoplasts is challenging with conventional cell sorters due to high sample pressures, shear stress and osmotic change which damage cells making them unusable for downstream applications. The ability of the WOLF G2 to enrich and sort protoplasts is essential to haploid plants regeneration.
Scatter Plots for Protoplasts Sorting.(A) Parent gate (chlorophyll positive events), (B) Subsequent child gate showing the three populations identified and sorted, which include a high chlorophyll-containing class, a low chlorophyll-containing class, and free chloroplasts from lysed pre-sorted cells. (C) Sorted high chlorophyll-containing protoplasts
Protoplast isolation and enrichment using the microfluidic WOLF® Cell Sorter
Cell isolation via gentle cell sorting is needed for workflows in plant biology including gene characterization and function, proteomics and transcriptomics, single-cell genomics, improved cell line development through gene engineering/CRISPR-Cas9 editing, and breeding.
Microspores are crucial in plant breeding programs and genetic studies, but their delicate nature requires specialized techniques for isolation and sorting. The WOLF G2 offers a solution with its gentle microfluidic technology, allowing for precise and efficient sorting of microspores while maintaining their viability and integrity. This enables researchers to obtain pure and homogeneous populations of microspores, facilitating downstream analyses and advancing research in plant genetics and breeding.
Sorting strategy for induced (embryogenic) Brassica napus microspores. Microspores were separated using estimated cell size (FSC-H), internal complexity/granularity (BSC-H), and green autofluorescence (FL1, B525-H) all excited by the 488nm laser. (Left) Subsequent child gate selecting for green auto fluorescent positive events (gated to singlets). (Right) a histogram representation of the green positive events within the child gate “singlets.”
Regeneration of Plant Embryogenic Microspores Enriched with the WOLF G2® Gentle Cell Sorter
Double haploid (DH) technology has become an integral component of commercial breeding programs in many agriculturally important crops. The primary advantage of this technology is the production of homozygous haploid inducer lines in an accelerated manner.
Sorting nuclei on the WOLF G2 is an important part of omics workflows and lower-throughput ploidy analyses. The WOLF G2 excels in preserving nuclei integrity throughout the sorting process, ensuring improved sequencing reads and accurate ploidy results. Unlike traditional cell sorters, the WOLF G2's gentle microfluidic technology delicately handles nuclei, minimizing cellular stress and maximizing the quality of sorted samples.
Sorting Strategy for plant nuclei. (Left) A representative sample of Roma tomato plant leaf nuclei Pre-sort and Post-sort enrichment. (Right) Plant debris from tomato and green bell peppers were considerably reduced post-sort, with an average 3-fold decrease.
Plant Nuclei Enrichment and Debris Removal with the WOLF Cell Sorter
Plant research has led to novel discoveries in health, agriculture and the environment. Flow sorting plant cells (protoplasts) can be challenging due to their fragility and large size. In addition, some downstream applications such as single-cell DNA sequencing have cell size limitations.
Gentle enrichment of plant protoplasts and nuclei using a microfluidic cell sorter
Cell isolation via gentle cell sorting is needed for numerous biological workflows in plant biology including gene characterization and function, proteomics and transcriptomics, single-cell genomics, improved cell line development through gene engineering/CRISPR-Cas9 editing, and breeding.
Sorting microalgae presents unique challenges due to their small size and delicate nature. The WOLF G2, equipped with microfluidic technology, offers a solution by providing gentle sorting of microalgae. This instrument allows researchers to efficiently isolate and purify microalgal cells, ensuring high viability and integrity post-sorting. With the WOLF G2, scientists can overcome the complexities of sorting microalgae and advance research in areas such as biofuel production, bioremediation, and bioprospecting for novel compounds.
Chlamydomonas reinhardtii sorting strategy. (Left) Algal cells were separated using chlorophyll autofluorescence (FL5, B706) and GFP positive expression (FL2, B525). (Right) Bulk enrichment of GFP positive Chlamydomonas reinhardtii as a function of GFP positive target ratio in unsorted population.
Enrichment and growth of transgenic microalgae using a gentle microfluidic cell sorter
Microalgae are a diverse group of organisms that play critical ecological roles as primary producers and contribute substantially to aquatic photosynthesis, which generates ~50% of the oxygen present in the earth’s biosphere. Microalgae are also increasingly leveraged in biotechnology applications for renewable fuels, food, nutraceuticals, and other bioproducts.
