Stem Cell Research Is Complex Isolating Them Doesn't Have To Be
The WOLF G2 offers precise and gentle cell sorting to handle delicate stem cells. Its microfluidic technology minimizes cell stress during sorting to preserve viability and integrity for regenerative medicine, disease modeling, or drug development. Whether you're working with pluripotent stem cells, hematopoietic stem cells, or mesenchymal stem cells, the WOLF G2's versatility allows you to adapt to the specific requirements of your research.
What Customers Say
Read firsthand accounts of how our cell sorting solutions are transforming scientific discoveries.
Right now, the best technology out there for [single stem cell cloning] is the WOLF.
Pluristyx
Single cell clonal outgrowth is “hovering at 45% with the WOLF, whereas with [a droplet based cell sorter] we maxed out at about 35%".
Large Pharma Scientist
The WOLF is easier for a non-crazy engineer to understand and use.
Mt. Sinai
Keep Your Stem Cells Happy
Experience gentle cell sorting with the NanoCellect's family of sorters. Elevate your research in stem cells and fragile cell populations.
Best-In-Class Clonal Outgrowth
The WOLF's low pressure and gentle microfluidics greatly reduce shear stress during single cell sorting resulting in higher number of clones per plate.
Sustained Pluripotency
Gentle microfluidic technology preserves the integrity of iPSCs during sorting, reducing unwanted stem cell differentiation.
Sterile Cell Sorting
The WOLF's sterile microfluidic cartridges prevent cross-contamination and eliminate aerosols, keeping you and your samples safe.
Simple and Compact
Our cell sorters are some of the most compact available. Intuitive and easy-to-use software allows any scientist to operate them. No expert required.
Flexible Media Compatibility
Traditional PBS sheath fluid isn't always preferred for many cell types. The WOLF G2 has the unique capability of sorting cells in their favored media.
Small and Mighty
No bulky fluidic cart or lines brings cell sorting right to your benchtop, boat, submarine or wherever science takes you.
iPSC Gene Editing
Genetic engineering methods that use viral, electroporation, lipofection or nanoparticles to introduce CRISPR, transposon, and other approaches are imperfect and require downstream selection of the edited cells. The WOLF G2 and N1 Single-Cell Dispenser lead the pack with gentle, sterile, and easy-to-use microfluidic technology to carry edited cells forward for monoclonal expansion and karyotyping. Bring the sorter to your cells by placing the WOLF and N1 on your bench or in your biosafety cabinet and sort on your cells’ schedule.
Using human induced pluripotent stem cells and CRISPR/Cas9 genome editing
In this study, we partnered a well characterized neurotypical human induced pluripotent stem cell (hiPSC) line with CRISPR-Cas9 genome editing to generate monogenic ASD hiPSC disease models.
WOLF G2 and N1 Single-Cell Dispenser: A Reliable Cloning Solution
The strength of iPSC models has been confirmed by recent FDA approvals of drugs to begin clinical trials based on iPSC-derived organoid data—replacing animal models. But reprogramming fibroblasts to iPSCs, genetically engineering the cells, and driving pluripotent cells to specific lineages can be an inefficient processes. The WOLF G2 Cell Sorter and N1 Single-Cell Dispenser differentiate themselves in single-cell cloning performance. The WOLF preserves pluripotency with gentle, low-pressure, and sterile microfluidic sorting. The N1 rapidly dispenses single cells into 96- or 384-well plates.
SSEA-4 and TRA-1-60 iPSC surface markers are used to sort iPSCs into 96-well plates. Figure on the right show iPSC clonal outgrouth after sorting with the WOLF.
Isolating Induced Pluripontent Stem Cells by using the WOLF G2 to Sort Surface Markers
Designed to streamline and expedite the generation of genome-edited iPSCs.
Maintaining High Viability of Unique and Fragile Cell Types With Gentle, Microfluidic Cell Sorting
Dive into the realm of preserving delicate cell viability with NanoCellect's insightful webinar on maintaining high viability of unique and fragile cell types.
