Stem Cell Application for the WOLF G2 Cell Sorter

Stem Cell Research Is Complex,
Isolating Them Doesn't Have To Be

The WOLF G2 offers precise and gentle cell sorting to handle delicate stem cells. Its microfluidic technology minimizes cell stress during sorting to preserve viability and integrity for regenerative medicine, disease modeling, or drug development. Whether you're working with pluripotent stem cells, hematopoietic stem cells, or mesenchymal stem cells, the WOLF G2's versatility allows you to adapt to the specific requirements of your research.

What Customers Say

Hear firsthand accounts of how our cell sorting solutions are transforming scientific discoveries.

Right now, the best technology out there for [single stem cell cloning] is the WOLF.

Pluristyx

Single cell clonal outgrowth is “hovering at 45% with the WOLF, whereas with [a droplet based cell sorter] we maxed out at about 35%".

Large Pharma Scientist

The WOLF is easier for a non-crazy engineer to understand and use.

Mt. Sinai

Keep Your Stem Cells Happy

Best-in-class Clonal Outgrowth

Gentle microfluidics eliminate shear stress during cell sorting for a higher number of clones per plate.

Sustained Pluripotency

Gentle microfluidic technology preserves the integrity of iPSCs during sorting, reducing unwanted stem cell differentiation.

Sterile Stem Cell Sorting

Sterile microfluidic cartridges prevent cross-contamination and eliminate aerosols, keeping you and your samples safe.

For Any Scientist

Intuitive design with easy-to-use software requires minimal user training. No expert required.

Flexible Media Capabilities

Traditional PBS sheath fluid isn't always prefered by stem cells. The WOLF G2 can sort stem cells in custom media.

Small and Mighty

No bulky fluidic cart or lines brings cell sorting right to your benchtop, boat, submarine or wherever science takes you.

iPSC Gene Editing

Genetic engineering methods that use viral, electroporation, lipofection or nanoparticles to introduce CRISPR, transposon, and other approaches are imperfect and require downstream selection of the edited cells. The WOLF G2 and N1 Single-Cell Dispenser lead the pack with gentle, sterile, and easy-to-use microfluidic technology to carry edited cells forward for monoclonal expansion and karyotyping. Bring the sorter to your cells by placing the WOLF and N1 on your bench or in your biosafety cabinet and sort on your cells’ schedule.

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iPSC Gene Editing isolation with the WOLF G2 Cell Sorter

Identification of iPSC (left) followed by the detection and isolation of single GFP-positive iPSCs in CRISPR/Cas9 gene editing protocols

Techniques for CRISPR/Cas9-Facilitated Genome Editing and Single-Cell Cloning of hPSCs

Induced pluripotent stem cells (iPSCs) are versatile in vitro model systems for studying human disease mechanisms directly in patient-derived cells. CRISPR/Cas9-faciliated genome editing has enabled efficient genetic manipulations for many mammalian cell

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Using human induced pluripotent stem cells and CRISPR/Cas9 genome editing

In this study, we partnered a well characterized neurotypical human induced pluripotent stem cell (hiPSC) line with CRISPR-Cas9 genome editing to generate monogenic ASD hiPSC disease models.

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The WOLF G2 is an invaluable tool for Stem Cell Research

The WOLF G2 also enables researchers to sort and dispense individual stem cells directly into well plates, a process essential for clonal isolation and downstream analyses. With its user-friendly design and exceptional reliability, the WOLF G2 simplifies the complex task of stem cell sorting, making it an indispensable asset for advancing stem cell research.

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Stem Cell Cloning

WOLF G2 and N1 Single-Cell Dispenser: A Reliable Cloning Solution.

The strength of iPSC models has been confirmed by recent FDA approvals of drugs to begin clinical trials based on iPSC-derived organoid data—replacing animal models. But reprogramming fibroblasts to iPSCs, genetically engineering the cells, and driving pluripotent cells to specific lineages can be an inefficient processes. The WOLF G2 Cell Sorter and N1 Single-Cell Dispenser differentiate themselves in single-cell cloning performance. The WOLF preserves pluripotency with gentle, low-pressure, and sterile microfluidic sorting. The N1 rapidly dispenses single cells into 96- or 384-well plates.

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iPSC and Stem Cell Cloning with the WOLF G2 Cell Sorter

SSEA-4 and TRA-1-60 iPSC surface markers are used to sort iPSCs into 96-well plates. Figure on the right show iPSC clonal outgrouth after sorting with the WOLF.

Isolating Induced Pluripontent Stem Cells by using the WOLF G2 to Sort Surface Markers

Designed to streamline and expedite the generation of genome-edited iPSCs.

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Gentle Sorting Accelerates Generation of Genome-Edited iPSCs

The WOLF yields a single-cell plating efficiency of up to 93% which is significantly more efficient than non-sorting methods.

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“Cutting the Mustard” with Induced Pluripotent Stem Cells:

Where the self-renewal property has unfolded the mysteries of regeneration, i.e., stem cells.

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Maintaining High Viability of Unique and Fragile Cell Types With Gentle, Microfluidic Cell Sorting

Dive into the realm of preserving delicate cell viability with NanoCellect's insightful webinar on maintaining high viability of unique and fragile cell types.

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