Up to Two Lasers and Nine Colors

Healthy Cells. Broad Capabilities. Better Science.
The WOLF was originally created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells effectively, easily, and of high quality. Now, as science continues to move forward into increasingly complex realms, the WOLF is moving forward with it. The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing. Single-cell sorting can be completed in 96- or 384-well plates when using the WOLF G2 in conjunction with the N1 Single-Cell Dispenser. This flexibility in performance, along with the additional abilities of the second laser, makes it ideal for use in many different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, immunology, infectious disease, basic research, and more.

Product Features
Sample Input
1.5 and 5.0 mL tubes
Sheath Input
50 mL Conical Tubes
Sheath Fluid
PBS or Buffer of Choice
Sheath Fluid Usage
9.6 mL/Hour
Sample Flow Rate
24 ul/Minute
Sheath Flow Rate
160 uL/Minute
Dead Volume
50 uL
Minimum Sample Volume
150 uL
Tubing Diameter (Inner)
200 to 500 um
Flow Cell
200 x 70 um
Sample Pressure
< 2psi
Cartridge based fluidics
NanoCellect utilizes unique and proprietary microfluidic sorting cartridges for bulk sorting and single-cell dispensing. The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many samples where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.
Download brochureExcitation
488 only or 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm
Laser Profile
25 x 27 µm
Scatter Detection
Forward (0 Degrees, +/-15)
Back (180 Degrees, +/-15)
Emission Detection
See WOLF G2 Configuration Guide
Optical Alignment
Fixed alignment, no customer maintenance required
Product Specs Table Optics
The WOLF G2 uses laser-based flow cytometry technology for detecting and sorting a variety of cell types, including mammalian, plant, fungal, and bacterial cells. The WOLF G2 cell sorter utilizes typical Forward- (FSC) and Back- (BSC) scatter detectors—as well as 3 different laser configurations to broaden its capabilities. In combination with the N1 single cell dispenser, scientists can complete both bulk and single-cell sorting. A simple cytometer, with powerful impact, allows researchers to sort their own cells on their own bench.

Software
- WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
- After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
- Key tools like automatic compensation and index sorting, are streamlined and included.
- When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
- Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing through the WOLFViewer software.
Scatter sensitivity
< 1.5 μm by FSC or BSC
Scatter Resolution
Resolves lymphocytes, monocytes, and granulocytes
Fluorescence sensitivity
< 200 MESF FITC (using 488 nm laser)
< 250 MESF PE (using 561 nm laser)
Fluorescence Resolution
8 peak separation with SPHERO™️
Rainbow Calibration Particles
Analysis Speed
> 5,000 events/second
Sorting
1- and 2-way
Back-to-back Sorting Speed
200 events per second
Absolute Counts
Yes
Volumetric Analysis
Yes
Warm-up Time
Less than 1 minute
Sorting Purity
Up to 99% purity
Sorting Output
1.5 mL and 5.0 mL, 96- and 384-well plates
Performance
The WOLF G2’s performance specifications allow users to complete various types of sorts for a variety of applications that may be occurring in their lab, for the ultimate in flexibility. The WOLF G2 cell sorter can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 μm, and up to 60 μm. Users can expect a sorting speed of up to 200 events per second maximally. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.
WOLF Dimensions
14.8H x 18.0W x 13.6D in (37.6H x 45.8W x 34.5D cm)
WOLF Weight
54 lbs (24.5kg)
WOLF Electrical
AC Input: 100-240V, 50/60Hz, 0.5A
N1 Dimensions
8.42W x 6.5H x 8.34D in (21.4 x 16.5 x 20.9)
N1 Weight
5.5 lbs / 2.5 kg
N1 Electrical
DC Input: 24V 1A
Physical Dimensions
The WOLF G2 cell sorter is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets. At 2 cubic feet and 54 pounds, researchers will not need to worry about the system taking up precious lab space.
Applications Overview
The NanoCellect WOLF G2 cell sorter is a powerful tool used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The WOLF G2 is designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The WOLF G2 cell sorter is ideal for tasks like cell line development and sorting plant protoplasts,
as well as for monoclonal outgrowth of iPSCs and microalgae.
Application Areas
View all applications
Explore the power of modern and simple cell sorting in many applications.
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