Up to Two Lasers and Nine Colors
Healthy Cells. Broad Capabilities. Better Science.
The WOLF Cell Sorter, born out of a team’s determination to tackle the challenges of biological research, has evolved alongside scientific progress with introduction of the WOLF G2 Cell Sorter pushing the boundaries of gentle benchtop microfluidic cell sorting. Equipped with two lasers and up to nine colors, it empowers researchers with expanded capabilities while maintaining user-friendly workflows for bulk sorting or precise single-cell dispensing. Leveraging the N1 Single-Cell Dispenser, the WOLF G2 Cell Sorter facilitates single-cell sorting into 96- or 384-well plates. This remarkable flexibility, combined with the enhanced functionality of the second laser, makes it a go-to solution for diverse fields like single-cell genomics, cell line engineering, gene editing, antibody discovery, immunology, infectious disease, plant biology, basic research, and more. Experience the future of advanced cell manipulation with the WOLF G2 Cell Sorter and unleash the potential of your research. Visit our webpage for further details on cell line development, single cell dispensing, and single cell isolation.
1.5 and 5.0 mL tubes
50 mL Conical Tubes
PBS or Buffer of Choice
Sheath Fluid Usage
Sample Flow Rate
Sheath Flow Rate
Minimum Sample Volume
Tubing Diameter (Inner)
200 to 500 um
200 x 70 um
Cartridge based fluidics
NanoCellect’s innovative approach to cell sorting encompasses both bulk and single-cell sorting through its unique and exclusive microfluidic cartridges. The microfluidic sorting cartridges operate at less than 2 psi to enable gentle sorting that preserves cellular viability and RNA integrity, as well as eliminate aerosol generation. With a 100% disposable fluidic pathway, these cartridges are particularly advantageous for laboratories handling numerous samples, mitigating concerns related to cross-contamination and biohazard cleanup. The combination of zero aerosol generation and the sterile microfluidic sorting cartridges provides an end-to-end fluidics path, that delivers one of the safest sorting experiences available. Additionally, these cartridges offer remarkable flexibility, allowing users to sort cells using their preferred buffers and media while consuming only approximately 50 mL of sheath fluid per day. Experience the power of single-cell dispensing, cell line engineering, and single-cell isolation with NanoCellect’s cutting-edge microfluidic sorting cartridges.Download brochure
488 only or 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm
25 x 27 µm
Forward (0 Degrees, +/-15)
Back (180 Degrees, +/-15)
See WOLF G2 Configuration Guide
Fixed alignment, no customer maintenance required
Product Specs Table Optics
The WOLF G2 uses laser-based flow cytometry technology for detecting and sorting a variety of cell types, including mammalian, plant, fungal, and bacterial cells. The WOLF G2 cell sorter utilizes typical Forward- (FSC) and Back- (BSC) scatter detectors—as well as 3 different laser configurations to broaden its capabilities. In combination with the N1 single cell dispenser, scientists can complete both bulk and single-cell sorting. A simple cytometer, with powerful impact, allows researchers to sort their own cells on their own bench.
- WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
- After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
- Key tools like automatic compensation and index sorting, are streamlined and included.
- When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
- Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing through the WOLFViewer software.
< 1.5 μm by FSC or BSC
Resolves lymphocytes, monocytes, and granulocytes
< 200 MESF FITC (using 488 nm laser)
< 250 MESF PE (using 561 nm laser)
8 peak separation with SPHERO™️
Rainbow Calibration Particles
> 5,000 events/second
1- and 2-way
Back-to-back Sorting Speed
200 events per second
Less than 1 minute
Up to 99% purity
1.5 mL and 5.0 mL, 96- and 384-well plates
The WOLF G2’s performance specifications allow users to complete various types of sorts for a variety of applications that may be occurring in their lab, for the ultimate in flexibility. The WOLF G2 cell sorter can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 μm, and up to 60 μm. Users can expect a sorting speed of up to 200 events per second maximally. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.
14.8H x 18.0W x 13.6D in (37.6H x 45.8W x 34.5D cm)
54 lbs (24.5kg)
AC Input: 100-240V, 50/60Hz, 0.5A
8.42W x 6.5H x 8.34D in (21.4 x 16.5 x 20.9)
5.5 lbs / 2.5 kg
DC Input: 24V 1A
The WOLF G2 cell sorter is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets. At 2 cubic feet and 54 pounds, researchers will not need to worry about the system taking up precious lab space.
The WOLF is a technology that enables small companies to do things they could not do before.” “The microfluidics capability is something that I have been excited about. The nature of how the WOLF works is being extremely attractive to us. Some of the experiments we’d like to do in the future could not be done on any other device.Justin Chartron, Ph.D. Protabit LLC
Neurons are extremely delicate so the selection step should be the least disturbing of all if we want to have reliable results. When we tried the WOLF, we were extremely surprised that our cells were very healthy and viability was above 90%, sometimes above 95% which is very impressive.Dorota Skowronska-Krawczyk, M.Sc.,Ph.D. University of California Irvine
A great machine that has helped us take our cell-engineering pipeline to the next level!Ronan O'Connell Rice University
The NanoCellect team and technical specialist have been awesome. After two test runs with very involved training/assistance, we have been very pleased with the results. The equipment is intuitive and the sorts have been very clean.B. Davenport, Ph.D. University of Colorado
NanoCellect Customer Support team has been fantastic in helping us get up and running. The instrument is a helpful tool in our cell line development workflow.Douglas Austen Takeda Pharmaceuticals
The NanoCellect customer support team is really helpful, knowledgeable and efficient; the WOLF is a great tool in our stem cell research workflowHelen Miranda, Ph.D. Case Western Reserve University
The NanoCellect WOLF G2 cell sorter is a powerful tool used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The WOLF G2 is designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The WOLF G2 cell sorter is ideal for tasks like cell line development and sorting plant protoplasts,
as well as for monoclonal outgrowth of iPSCs and microalgae.
Explore the power of modern and simple cell sorting in many applications.View content
Learn more about a variety of scientific topics related to flow cytometry.Go to blog
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