Up to Two Lasers and Nine Colors
Healthy Cells. Broad Capabilities. Better Science.
The WOLF was originally created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells effectively, easily, and of high quality. Now, as science continues to move forward into increasingly complex realms, the WOLF is moving forward with it. The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing. Single-cell sorting can be completed in 96- or 384-well plates when using the WOLF G2 in conjunction with the N1 Single-Cell Dispenser. This flexibility in performance, along with the additional abilities of the second laser, makes it ideal for use in many different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, immunology, infectious disease, basic research, and more.
1.5 and 5.0 mL tubes
50 mL Conical Tubes
PBS or Buffer of Choice
Sheath Fluid Usage
Sample Flow Rate
Sheath Flow Rate
Minimum Sample Volume
Tubing Diameter (Inner)
200 to 500 um
200 x 70 um
Cartridge based fluidics
NanoCellect utilizes unique and proprietary microfluidic sorting cartridges for bulk sorting and single-cell dispensing. The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many samples where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.Download brochure
488 only or 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm
25 x 27 µm
Forward (0 Degrees, +/-15)
Back (180 Degrees, +/-15)
See WOLF G2 Configuration Guide
Fixed alignment, no customer maintenance required
Product Specs Table Optics
The WOLF G2 uses laser-based flow cytometry technology for detecting and sorting a variety of cell types, including mammalian, plant, fungal, and bacterial cells. The WOLF G2 cell sorter utilizes typical Forward- (FSC) and Back- (BSC) scatter detectors—as well as 3 different laser configurations to broaden its capabilities. In combination with the N1 single cell dispenser, scientists can complete both bulk and single-cell sorting. A simple cytometer, with powerful impact, allows researchers to sort their own cells on their own bench.
- WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
- After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
- Key tools like automatic compensation and index sorting, are streamlined and included.
- When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
- Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing through the WOLFViewer software.
< 1.5 μm by FSC or BSC
Resolves lymphocytes, monocytes, and granulocytes
< 200 MESF FITC (using 488 nm laser)
< 250 MESF PE (using 561 nm laser)
8 peak separation with SPHERO™️
Rainbow Calibration Particles
> 5,000 events/second
1- and 2-way
Back-to-back Sorting Speed
200 events per second
Less than 1 minute
Up to 99% purity
1.5 mL and 5.0 mL, 96- and 384-well plates
The WOLF G2’s performance specifications allow users to complete various types of sorts for a variety of applications that may be occurring in their lab, for the ultimate in flexibility. The WOLF G2 cell sorter can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 μm, and up to 60 μm. Users can expect a sorting speed of up to 200 events per second maximally. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.
14.8H x 18.0W x 13.6D in (37.6H x 45.8W x 34.5D cm)
54 lbs (24.5kg)
AC Input: 100-240V, 50/60Hz, 0.5A
8.42W x 6.5H x 8.34D in (21.4 x 16.5 x 20.9)
5.5 lbs / 2.5 kg
DC Input: 24V 1A
The WOLF G2 cell sorter is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets. At 2 cubic feet and 54 pounds, researchers will not need to worry about the system taking up precious lab space.
The WOLF is a technology that enables small companies to do things they could not do before.” “The microfluidics capability is something that I have been excited about. The nature of how the WOLF works is being extremely attractive to us. Some of the experiments we’d like to do in the future could not be done on any other device.Justin Chartron, Ph.D. Protabit LLC
Neurons are extremely delicate so the selection step should be the least disturbing of all if we want to have reliable results. When we tried the WOLF, we were extremely surprised that our cells were very healthy and viability was above 90%, sometimes above 95% which is very impressive.Dorota Skowronska-Krawczyk, M.Sc.,Ph.D. University of California Irvine
A great machine that has helped us take our cell-engineering pipeline to the next level!Ronan O'Connell Rice University
The NanoCellect team and technical specialist have been awesome. After two test runs with very involved training/assistance, we have been very pleased with the results. The equipment is intuitive and the sorts have been very clean.B. Davenport, Ph.D. University of Colorado
NanoCellect Customer Support team has been fantastic in helping us get up and running. The instrument is a helpful tool in our cell line development workflow.Douglas Austen Takeda Pharmaceuticals
The NanoCellect customer support team is really helpful, knowledgeable and efficient; the WOLF is a great tool in our stem cell research workflowHelen Miranda, Ph.D. Case Western Reserve University
The NanoCellect WOLF G2 cell sorter is a powerful tool used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The WOLF G2 is designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The WOLF G2 cell sorter is ideal for tasks like cell line development and sorting plant protoplasts,
as well as for monoclonal outgrowth of iPSCs and microalgae.
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