Simplified sample prep and higher quality results.
Single Cell Genomics
Our understanding of genomics & science, in general, is constantly changing. Scientists across the globe are constantly finding new ways to pioneer emerging fields and they require cutting edge tools and devices to do so. Our goal at NanoCellect was to create a cell-based assay technologies, like our benchtop flow cytometry, cell sorting and dispensing system, in order for these researchers to move forward in their scientific research.
Specifically within the field of molecular biology, the scientific study of genomics has progressed immensely over the last decade; however most of the human genome still remains to be explored and fully understood. While there are many approaches to tackling modern day medical issues and genomic analysis, single cell genomics allows for a more narrowed focus and the study of a specific cell where the data doesn’t come from a cell population average. Instead, the data from each of the individual cells (of a specific cell type) are measured. Prior to the growth of single cell genomics, the issue had been isolating the single cells of interest and easy amplification of the sample for study. For example, immune cells or progenitor cells that were not purified correctly did not result in quality genomic data or informative expression profiles. In order to fully understand specific cell types and how they interact on a cellular level, we must analyze the RNA & DNA sequencing data of each of these cells on an individual level. This can be useful for studying gene mutations (within tumor cells), gene regulation and gene expression. With technological innovations in the single cell field, it’s now easier than ever to prepare single cell samples with our benchtop cell sorting/dispensing system and gain insights with our easy-to-use single cell analysis tools.
Sample preparation is a critical (yet often underappreciated) step in single cell genomics, single-cell analysis for various fields of research. Many current methods of sample prep can risk damaging its cellular structure; however, NanoCellect’s microfluidic process allows for cell suspension in fluid to protect the cell’s integrity for the purposes of DNA & RNA single cell sequencing. When it comes to improvements in cell sample purity and viability, the following areas can benefit greatly:
- Next generation sequencing on Illumina or IonTorrent platforms
- Genomic library prep with 10X Genomics or Fluidigm systems
- Plate based RNA-seq kits from QIAGEN or Takara
Optimizing your single cell preparation can maximize your cell samples in two ways:
- By inputting the “right” cells with selection based on viability and other parameters if needed
- By utilizing sorting technology that leaves cells or nuclei intact.
That’s where the WOLF Cell Sorter comes in.
By selecting and enriching only the target individual cells of interest you can avoid the need to sequence and analyze dying cells, debris, and non-target cells—directly impacting your total cost per cell analyzed and allow researchers more time to plan experiments efficiently.
The NanoCellect WOLF Cell Sorter also provides accurate cell count of selected cells and eliminates the need for a cell counter or viability check prior to your analytic step. In addition, the WOLF provides an easy-to-use cell enrichment device and single cell dispenser that does not require expertise in flow cytometry. NanoCellect’s system is:
- Robust, portable, and sterile
- Safe for single cell DNA and RNA sequencing
- Efficient in single cell sorting and cloning operations
- Can be used in any lab without the need for dedicated hoods, venting, or expert personnel
The sterile, exchangeable fluidics system of the WOLF eliminates carry-over between samples and makes clean up a snap. When in doubt about how to optimize efficiencies in your lab, just ask yourself:
What is single cell sorting without WOLF? Cumbersome.
What is single cell sorting with WOLF? Ideal.
That’s the NanoCellect guarantee—that’s happy cells.