Techniques for CRISPR/Cas9-Facilitated Genome Editing and Single-Cell Cloning of hPSCs

Still hand-picking iPSC colonies? Consider gentle single-cell sorting. Join us for our mini webinar highlighting tips and techniques for sorting iPSC. We review:

• • Example workflows for fluorescence targeted CRISPR/Cas9 screening
  • Techniques and challenges when preparing iPSC for sorting
  • How gentle single-cell sorting will reduce your iPSC workload & simplify clonal selection

We discuss methods for sorting CRISPR/Cas9-edited iPSC. This discussion showcases work from our collaborators at the Human Stem Cell Core at Baylor College of Medicine, among others, to convey how using the WOLF cell sorter and N1 single-cell dispenser has enabled recent advancements.

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