ShopRevolutionizing Stem Cell Selection: A Label-free Approach Using the VERLO Image-Guided Cell Sorter
Abstract
In the evolving landscape of stem cell research, the ability to distinguish between undifferentiated and differentiated cells is paramount for advancing both basic science and therapeutic applications. The traditional reliance on fluorescent markers for cell sorting, although effective, introduces complexity and may affect cell viability and functionality. Our recent study leverages the VERLO™ Image-Guided Cell Sorter’s capabilities to address this challenge in a label-free manner, revolutionizing the process of stem cell selection. Utilizing imaging features and advanced analytics, the VERLO cell sorter enables the precise identification and separation of undifferentiated from differentiated human pluripotent stem cells without the need for fluorescent labels. This approach preserves cell integrity and eliminates the potential impacts of labeling on cell physiology. Our results demonstrate a significant advancement in stem cell research methodologies, offering a more efficient, reliable, and non-invasive alternative for cell sorting. We successfully applied this label-free sorting technique to isolate pure populations of undifferentiated stem cells, achieving high efficiency and viability. This method not only enhances the potential for scalable stem cell applications but also opens new avenues for research into cellular development, disease modeling, and regenerative medicine. The utility of the VERLO Image-Guided Cell Sorter in this context underscores its value as a critical tool for the next generation of stem cell research.
PST-016