✍🏼 Xinyue Du, Xiao Chen, Chiyuan Gao, Junbo Wang, Xiaoye Huo, Jian Chen

🏠 State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing , China

📑Biosensors (2025)

Abstract
This article reviews recent developments in flow cytometry that have a significant impact on both scientific research and clinical applications in the field of single-cell analysis, from the perspective of instrumentation and technical advances. As a starting point, this article investigates the latest state-of-the-art instruments of flow cytometry including different types in spectral, mass, imaging, nano, and label-free flow cytometry. A comparative analysis of the parameters and features of instruments from different companies elucidates the development trends in flow cytometry instrumentation. Following this, this article delves into cutting-edge technical advancements in flow cytometry. It summarizes the current research status of flow cytometry not only globally but also within China, highlighting emerging trends and innovations in the field. Finally, this article outlines future directions for the development of flow cytometry, indicating that each type of flow cytometry will follow its own trajectory toward achieving enhanced performance and broader applications in diverse fields.