Webinar Series

High Viability Cell Sorting for Single-Cell 3’ RNA-seq

We highlight the importance of cell sample preparation in order to obtain good quality sequencing data with 3’ RNA-Seq. We will review:

  • The challenges for obtaining high quality data for Single-Cell 3’RNA-seq
  • Why low-pressure cell sorting can help preserve cell viability and reduce noise in sequencing data
  • How cell sorting was used in neuronal gene expression studies
Sorting Fragile Primary Cells for In Vitro Expansion and Downstream Assays

We highlight the importance of gentle sorting techniques to isolate fragile primary cells. We will review:

  • The challenges of sorting fragile primary cells.
  • How low-pressure cell sorting permits the extraction of previously unsortable primary cells
  • How gentle isolation yields quality cells
Microfluidics-based Cell Sorting to Purify Neutrophils with High Viability

Neutrophils have a short half-life and are extremely prone to degradation during processing. We will review:

  • The function and characteristics of neutrophils
  • Why neutrophils are notoriously difficult to sort
  • How to prepare fresh neutrophils for sorting
Leveraging sterile cell sorting of biohazard samples

We highlight the current challenges associated with research involving biohazard samples for cell sorting applications. We will review:

  • The safety challenges for researchers
  • The difficultly of using flow core facilities and shared equipment
  • How bench-top microfluidic-based cell sorting offers a flexible alternative
Challenges Sorting Microbial Cells? Consider Microfluidics-Based Cell Sorting

We showcase user-case to sort microbes and how to remove other sub-cellular organelles from your nuclear prep. Join us to learn:

  • Unique consideration for analysis and sorting of small cells
  • How single-cell sorting can streamline your discovery process
  • Advantages of the disposable fluidics for microbial cell sorting
Achieve higher clone quality and quantity by using single cell sorting

We highlight the advantages that cell sorting provides for developing monoclonal cell lines over limiting dilution. We will review:

  • What is limiting dilution, and what are its respective challenges and limitations
  • How cell sorting can enhance cloning efficiency, while reducing time, costs, and resources
  • How to increase the quality of clonal outcome by using fluorescent cell sorting
Cell Sorting Sample Preparation for Single Cell 10x Genomics

We discuss the importance of cell sample preparation in order to obtain optimal sequencing results for 10x Genomics applications. We review:

  • The challenges in obtaining high quality data for single cell genomic applications
  • Why low-pressure cell sorting can help preserve cell viability and reduce background noise in your sequencing results
  • How cell sorting can dramatically improve the quality of your sequencing results
Cell Sorting for COVID-19 Research

We discuss the use of FACS in various aspects of COVID-19 research and how the availability of microfluidic-based cell sorting provides a safe and sterile solution for researcher working on COVID-19. We will review:

  • Biomarker analysis of immune cells and biomarkers of COVID-19 patients
  • Immune cells response characterization using single cell genomics
  • Immune response profiling of COVID-19 patient
Techniques for single-cell iPSC cloning in CRISPR/Cas9 workflows

Still hand-picking iPSC colonies? Consider gentle single-cell sorting. Join us for our mini webinar highlighting tips and techniques for sorting iPSC. We review:

  • Example workflows for fluorescence targeted CRISPR/Cas9 screening
  • Techniques and challenges when preparing iPSC for sorting
  • How gentle single-cell sorting will reduce your iPSC workload & simplify clonal selection
Interrogating the SARS-CoV-2 specific immune response with single cell resolution: End to end workflow

SARS-CoV-2, responsible for COVID-19 disease, has erupted a unifying collaborative response effort to elucidate and define protective immunity to guide development of therapeutics and vaccines.

  • How dCODE™ Dextramer® technology adds a unique DNA barcode to the MHC Dextramer® reagent, allowing detection of antigen-specific T cells
  • Why sample preparation for genomic applications using microfluidic-based cell sorting improves the sequencing data of downstream applications
  • How combining dCODE Dextramer reagent with the 5’ immune solution from 10X Genomics can help COVID-19 research move forward
Microfluidic-based cell sorting to better sample preparation for 10x Genomics applications

We discuss how using cell sorting upstream of single cell RNA sequencing enables you to sensitively and efficiently identify and characterize immune cell interactions. Highlighting a study using cell sorting to identify a small fraction of CD8+ T cells bound to peptide-MHCs, we’ll explore how characterizing only these T cells cells of interest increased the potential to detect rare T cell types and reduced sequencing costs by eliminating the need to sequence all species present in the original sample.

Combining Microbubbles and Cell Sorting for Quick, Gentle Isolation of Unique Cell Populations

Sample preparation is a critical step in any scientific workflow. To ensure high quality results from downstream applications such as single-cell sequencing, it is imperative that the sample be pure and unaltered by harsh retrieval techniques. We highlight the benefits of combining Akadeum’s Human CD4+ T Cell Isolation Kit ahead of sorting cells with the microfluidic-based WOLF cell sorter for regulatory T cells enrichment.